The Comparison of Ketokonazol and Hypochlorous Acid (Hocl) Applications for the Treatment of The Fungal Infections (Dermatophytosis)




Fungi, Therapy, HOCl, Cat, Dog


Dermatophytosis is a mycotic disease of the skin that is resistant to treatment. The aim of this study was to investigate the treatment efficacy of a novel antimicrobial agent, Hypochlorous Acid (HOCL), on dermatophytosis of cats and dogs, in comparison with Ketaconazole. In this study, a total of 76 animals (26 cats and 50 dogs) without any disease other than skin fungal infection after clinical, hematological, biochemical, microscopic and Wood's lamp examinations were used. Subjects were randomly assigned to two equal treatment groups within their own species as HOCL: HP and Ketaconazole: KT. Naked eye inspection results were collected on the 8th, 11th and 15th days of all patients. The study was terminated on the 15th day by collecting the Wood's lamp and microscopic examination data together with the last inspection finding. Findings were analyzed statistically with chi-square and CART (Classification and Regression Tree) algorithm test. Inspection results of the treatment groups exhibited significant recovery over time (day 8, 11 and 15) for both species (p<0.05). However, the 15th day Wood‘s lamp and microscopic examination did not confirm the inspection findings. It was seen that the gender did not affect the results (p>0.05). According to the microscopic examination results, a significant statistical difference was observed between the HP and KT groups (p<0.05), but the same situation were not seen on the inspection and Wood‘s lamp examinations (p>0.05). As a result, it was concluded that HOCl has an effect on dermatophytosis of cat and dogs, although not as much as Ketaconazole, but further studies are needed to reveal the results more clearly.


Albrich JM, McCarthy CA, Hurst JK. 1981. Biological reactivity of hypochlorous acid: implications for microbicidal mechanisms of leukocyte myeloperoxidase. Proceedings of the National Academy of Sciences 78: 210–214.

Al-Janabi AAHS, Bashi AM. 2018. Development of a new synthetic xerogel nanoparticles of silver and zinc oxide against causative agents of dermatophytoses. The Journal of Dermatological Treatment 30: 283–287.

Alkan KK, Çiftçi MF, Yeşilkaya ÖF, Satılmış F, Tekindal MA, Alkan H. 2020. Pyometralı köpeklerde laktat dehidrogenaz, tam kan ve bazı serum biyokimya parametreleri arasındaki ilişkinin değerlendirilmesi. Eurasian Journal of Veterinary Sciences 36: 204–213.

AL-Khalidi F Q., Saatchi R, Burke D, Elphick H, Tan S. 2011. Respiration rate monitoring methods: A review. Pediatric Pulmonology 46: 523–529.

Anikar MJ, Bhadesiya CM, Gajjar PJ, Patel VA, Dadawala AI, Makwana PP, Patil DB. 2022. Evaluation of therapeutic protocols containing different antifungal agents to treat dermatophytosis in dogs. The Pharma Innovation Journal 11: 83–87.

Anonymous (Anonim). 2009. T.C. Çevre ve Orman Bakanlığı, Doğa Koruma ve Milli Parklar Genel Müdürlüğü Tarih: 25.06.2009, sayı:159/5316.

Aratani Y, Kura F, Watanabe H, Akagawa H, Takano Y, Suzuki K, Maeda N, Koyama H. 2000. Differential host susceptibility to pulmonary infections with bacteria and fungi in mice deficient in myeloperoxidase. The Journal of Infectious Diseases 182: 1276–1279.

Ateş FM. 2020. Water, salt, hypochlorous acid and infection protection. Bayburt Üniversitesi Fen Bilimleri Dergisi 3: 154–160.

Blazizza MFS, Rahal SC, Santos IFC, Silva BM, Ferreira GM, Oba E, Tsunemi MH, Takahira RK. 2021. Effects of a single session of whole-body vibration exercise on haematological and biochemical parameters, and serum cortisol levels in cats. Comparative Exercise Physiology 17: 1–6.

Block MS, Rowan BG. 2020. Hypochlorous acid: A review. Journal of Oral and Maxillofacial Surgery 78: 1461–1466.

Brescini L, Fioriti S, Morroni G, Barchiesi F. 2021. Antifungal combinations in Dermatophytes. Journal of Fungi 7: 727.

Choi FD, Juhasz ML, Mesinkovska NA. 2019. Topical ketoconazole: a systematic review of current dermatological applications and future developments. Journal of Dermatological Treatment 30: 760–771.

Çifci N. 2018. Retrospective evaluation of effects of vitamin D levels on skin diseases. Kocaeli Medical Journal 7: 47–54.

Endo EH, Makimori RY, Companhoni MVP, Ueda-Nakamura T, Nakamura CV, Dias Filho BP. 2020. Ketoconazole-loaded poly-(lactic acid) nanoparticles: Characterization and improvement of antifungal efficacy in vitro against Candida and dermatophytes. Journal de Mycologie Médicale 30: 101003.

Ertaş R, Kartal D, Utaş S. 2015. Çocuklarda yüzeysel mantar enfeksiyonlarının klinik değerlendirilmesi. Turkish Journal of Dermatology 9: 186–189.

Fam A, Finger PT, Tomar AS, Garg G, Chin KJ. 2020. Hypochlorous acid antiseptic washout improves patient comfort after intravitreal injection: A patient reported outcomes study. Indian Journal of Ophthalmology 68: 2439–2444.

Fuentes L. 2018. Efecto del ácido hipocloroso como alternativa terapéutica sobre la endometritis bovina posparto. Universidad Tecnica Ambato. Ciencias Agropecuarias, Tesis Midicina Veterinaria. Bachelor’s Thesis. 2018. Ambato - Ecuador.

García VJ, Márquez CO, Isenhart TM, Rodríguez M, Crespo SD, Cifuentes AG. 2019. Evaluating the conservation state of the páramo ecosystem: An object-based image analysis and CART algorithm approach for central Ecuador. Heliyon 5: e02701.

Gold MH, Andriessen A, Bhatia AC, Bitter Jr P, Chilukuri S, Cohen JL, Robb CW. 2020. Topical stabilized hypochlorous acid: The future gold standard for wound care and scar management in dermatologic and plastic surgery procedures. Journal of cosmetic dermatology 19: 270–277.

Goto K. 2015. Use of hypochlorous acid solution as a disinfectant in laboratory animal facilities. Annals of Clinical and Medical Microbiology 1: 1005.

Gray D, Foster K, Cruz A, Kane G, Toomey M, Bay C, Kardos P, Ostovar GA. 2016. Universal decolonization with hypochlorous solution in a burn intensive care unit in a tertiary care community hospital. American Journal of Infection Control 44: 1044–1046.

Hakim H, Thammakarn C, Suguro A, Ishida Y, Nakajima K, Kitazawa M, Takehara K. 2015. Aerosol disinfection capacity of slightly acidic hypochlorous acid water towards newcastle disease virus in the air: an in vivo experiment. Avian Diseases 59: 486–491.

Hammer O, Harper D, Ryan P. 2001. PAST: Paleontological Statistics software package for education and data analysis. Past 3.26 Oyvind Hammer, Palaeontologia Electronica 4(1): 9pp.

Hanedan B, Bi̇lgi̇li̇ A, Uysal MH. 2021. Kedi ve Köpeklerde deri mantar enfeksiyonlarinin insanlarda oluşturduğu sağlik riskleri, kontrol ve sağaltim seçenekleri. Icontech International Journal 5: 10–17.

Indarjulianto S, Yanuartono Y, Nururrozi A, Raharjo S, Ajiguna JC. 2020. Combination of systemic and topical treatment for feline dermatophytosis: A case report. Acta Veterinaria Indonesiana 8: 18–23.

Ishihara M, Murakami K, Fukuda K, Nakamura S, Kuwabara M, Hattori H, Fujita M, Kiyosawa T, Yokoe H. 2017. Stability of weakly acidic hypochlorous acid solution with microbicidal activity. Biocontrol Science 22: 223–227.

Joachim D. 2020. Wound cleansing: Benefits of hypochlorous acid. Journal of Wound Care 29: S4–S8.

Kanclerz P, Grzybowski A, Olszewski B. 2019. Low efficacy of hypochlorous acid solution compared to povidone-iodine in cataract surgery antisepsis. The Open Ophthalmology Journal 13: 29–33.

Kara T. 2020. Prarmetrik olmayan istatistik yöntemleri ders notları. Fen-Edebiyat Fakültesi İstatistik Bölümü: Yıldız Teknik Üniversitesi, İstanbul.

Karabulut H, Gülay MŞ. 2016. Antioksidanlar. MAE Vet Fak Derg 1: 65–76.

Karademir B. 2001. KAÜ Veteriner Fakültesi İç Hastalıkları Kliniklerine 1999 yılında kabul edilen hayvanların genel durumları. Journal of Faculty of Veterinary Medicine, Istanbul University 27: 377–383.

Katiraee F, Kosari YK, Soltani M, Shokri H, Minooieanhaghighi MH. 2021. Molecular identification and antifungal susceptibility patterns of dermatophytes isolated from companion animals with clinical symptoms of dermatophytosis. Journal of Veterinary Research 65: 175–182.

Kearns S, Dawson R. 2000. Cytoprotective effect of taurine against hypochlorous acid toxicity to PC12 cells. Advances in Experimental Medicine and Biology 483: 563–570.

Kelly W. 1984. Veterinary Clinical Diagnosis. Baillière Tindal, London.

Kim Y-R, Nam S-H. 2018. Comparison of the preventive effects of slightly acidic HOCl mouthwash and CHX mouthwash for oral diseases. Biomedical Research 29: 1718–23.

Kulak M, Gul F, Sekeroglu N. 2020. Changes in growth parameter and essential oil composition of sage (Salvia officinalis L.) leaves in response to various salt stresses. Industrial Crops and Products 145: 112078.

Łagowski D, Gnat S, Nowakiewicz A, Osińska M, Zięba P. 2019. The prevalence of symptomatıc dermatophytoses ın dogs and cats and the pathomechanısm of dermatophyte ınfectıons. Postępy Mikrobiologii - Advancements of Microbiology 58: 165–176.

Lee SH, Kim JW, Lee BC, Oh HJ. 2019. Age-specific variations in hematological and biochemical parameters in middle- and large-sized of dogs. Journal of Veterinary Science 21: e7.

Leite LL, Renato MB. 2022. Erythematous Plaque on the Groin and Buttocks. Cutis 109: E21–E23.

Lemon SC, Roy J, Clark MA, Friedmann PD, Rakowski W. 2003. Classification and regression tree analysis in public health: Methodological review and comparison with logistic regression. Annals of Behavioral Medicine 26: 172–181.

Mattei AS, Beber MA, Madrid IM. 2014. Dermatophytosis in small animals. SOJ Microbiology & Infectious Diseases 2: 1–6.

Moriello K. 2019. Dermatophytosis in cats and dogs: A practical guide to diagnosis and treatment. In Practice 41: 138–147.

Moriello KA. 2004. Treatment of dermatophytosis in dogs and cats: review of published studies. Veterinary Dermatology 15: 99–107.

Moriello KA. 2020. Dermatophytosis. In: Noli C, Colombo S (eds), Feline Dermatology. Cham: Springer International Publishing, London, UK. pp 265–296.

Nelson RW, Couto CG. 2019. Small Animal Internal Medicine - E-Book (6th Edition edn). Elsevier Health Sciences.

Neves JJA, Paulino AO, Vieira RG, Nishida EK, Coutinho SDA. 2018. The presence of dermatophytes in infected pets and their household environment. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 70: 1747–1753.

Nguyen T-V, Ichiki M. 2019. MEMS-Based Sensor for Simultaneous Measurement of Pulse Wave and Respiration Rate. Sensors 19: 4942.

Odorcic S, Haas W, Gilmore MS, Dohlman CH. 2015. Fungal Infections Following Boston Type 1 Keratoprosthesis Implantation: Literature Review and In Vitro Antifungal Activity of Hypochlorous Acid. Cornea 34: 1599–1605.

Romanowski EG, Yates KA, Romanowski JE, Mammen A, Dhaliwal DK, Jhanji V, Shanks RM, Kowalski RP. 2020. The Disinfection of Bacterial, Fungal, and Viral Contaminated Contact Lenses and Cases with Hypochlorous Acid. Investigative Ophthalmology & Visual Science 61: 413–413.

Rutala WA, Cole EC, Thomann CA, Weber DJ. 1998. Stability and bactericidal activity of chlorine solutions. Infection Control & Hospital Epidemiology 19: 323–327.

Sakarya S, Gunay N, Karakulak M, Ozturk B, Ertugrul B. 2014. Hypochlorous acid: an ideal wound care agent with powerful microbicidal, antibiofilm, and wound healing potency. Wounds 26: 342–350.

Salvatore S, Bramness JG, Røislien J. 2016. Exploring functional data analysis and wavelet principal component analysis on ecstasy (MDMA) wastewater data. BMC Medical Research Methodology 16: 81.

Sánchez TAC, García PAE, Zamora CIL, Martínez MA, Valencia VP, Orozco AL. 2014. Use of propolis for topical treatment of dermatophytosis in dog. Open Journal of Veterinary Medicine 4: 239.

Sav H 2017. Deri Hastalıklarında Mikolojik Tetkikler. Dermatoz 8: 1–6.

Siğirci BD, Meti̇ner K, Çeli̇k B, Kahraman BB, İki̇z S, Bağcigi̇l A funda, Özgür N yakut, Ak S. 2019. Dermatophytes Isolated From Dogs and Cats Suspected Dermatophytoses in Istanbul, Turkey Within A 15-Year-Period: An Updated Report. Kocatepe Veterinary Journal 12: 116–121.

Şimay T. 2014. Trikofitili sığırlarda trikofitozisin yaygınlığına göre serum çinko düzeyinin değerlendirilmesi. Yüksek Lisans Tezi. Kafkas Üniversitesi, Sağlık Bilimleri Enstitüsü, Veteriner İç Hastalıkları Anabilim Dalı. Kars.

Şimay T, Karademir B. 2015. Trikofitili Sığırlarda Trikofitozisin Yaygınlığına Göre Serum Çinko Düzeyinin Değerlendirilmesi. 11. Veteriner İç Hastalıkları Kongresi, 21-23 Mayıs, sf: 106, Samsun-Türkiye.

Şimay T, Karademir B. 2020. Evaluation of Serum Zinc Levels in Cattle with Trichophytosis According to Extensiveness of Trichophytosis. Turkish Journal of Agriculture-Food Science and Technology 8: 2416–2420.

Tabachnick BG, Fidell LS. 2013. Using multivariate statistics (Sixty edn). Pearson Inc. Boston.

Tan CL, Knight ZA. 2018. Regulation of Body Temperature by the Nervous System. Neuron 98: 31–48.

Taşçene N, Karagül H. 2008. Diyabetli köpeklerde kan HbA1C düzeyleri. Ankara Üniversitesi Vetereiner Fakültesi Dergisi 55: 75–78.

Türkmen D, Türkoğlu G. 2019. Erythrasma frequency in patients with interdigital maceration. Turkderm-Turk Arch Dermatol Venereology 53: 97–100.

Wang L, Bassiri M, Najafi R, Najafi K, Yang J, Khosrovi B, Hwong W, Barati E, Belisle B, Celeri C. 2007. Hypochlorous Acid as a Potential Wound Care Agent. Journal of Burns and Wounds 6: e5.

Wisal G. 2018. An over view of canine dermatophytosis. South Asian Journal of Research in Microbiology 2: 1–16.

Yapicier Ö, Şababoğlu E, Öztürk D, Pehli̇vanoğlu F, Kaya M, Türütoğlu H. 2017. Kedi ve Köpeklerden Dermatofitlerin İzolasyonu. Veterinary Journal of Mehmet Akif Ersoy University 2: 125–130.

Yokoi S, Sekiguchi M, Kano R, Kobayashi T. 2010. Dermatophytosis caused by Trichophyton rubrum infection in a dog. Japanese Journal of Veterinary Dermatology 16: 211–215.




How to Cite

Babur, M., & Karademir, B. (2023). The Comparison of Ketokonazol and Hypochlorous Acid (Hocl) Applications for the Treatment of The Fungal Infections (Dermatophytosis). Turkish Journal of Agriculture - Food Science and Technology, 11(4), 791–798.



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