Estimation of Relationship Between In Situ and In Vitro Rumen Protein Degradability of Extruded Full Fat Soybean

Authors

  • Arzu Erol Tunç International Center for Livestock Research and Training, 06852 Ankara
  • Yusuf Cufadar Department of Animal Science, Faculty of Agriculture, Selçuk University, 42130 Konya
  • Sema Yaman Department of Animal Production and Technologies, Faculty of Agricultural Sciences and Technologies, Niğde Ömer Halisdemir University, 51240 Niğde

DOI:

https://doi.org/10.24925/turjaf.v5i10.1237-1242.1426

Keywords:

In situ, in vitro, enzymatic method, Streptomyces griseus, protein degradability

Abstract

The objectives of this study were to estimate the protein degradability of extruded full fat soybean (ESB) by in situ (nylon bag) and in vitro enzymatic method and to develop an equation in order predict in situ degradability from in vitro values. In the study enzymatic technique; hydrolysis after 1 h (INV1) and after 24 h (INV24) by a purified protease extracted from Streptomyces griseus in a borate-phosphate buffer at pH 8 was used as in vitro method. Relationship between in situ effective protein degradability (INSE) and in vitro degradability after 1 and 24 hours incubations (INV1 and INV24) were determined. In situ protein degradability was measured at 0, 2, 4, 8, 16, 24, and 48 and at 72 h incubations in the rumen of 3 Holstein cows. In the study INSE, INV1 and INV24 were determined as 58.05, 20.24 and 41.46% respectively. Despite there were differences between in situ and in vitro protein degradability values, correlation coefficients between in situ and in vitro protein degradability of ESB were high and regression equations for estimation of in situ from in vitro were found significant. As conclusion in vitro enzymatic protein degradability (INV1 and INV24) can be used for estimation of in situ effective protein degradability of extruded full fat soybean.

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Published

02.10.2017

How to Cite

Erol Tunç, A., Cufadar, Y., & Yaman, S. (2017). Estimation of Relationship Between In Situ and In Vitro Rumen Protein Degradability of Extruded Full Fat Soybean. Turkish Journal of Agriculture - Food Science and Technology, 5(10), 1237–1242. https://doi.org/10.24925/turjaf.v5i10.1237-1242.1426

Issue

Section

Animal Production