Molecular Detection and Phylogenetic Analysis of Toxoplasma gondii in Minced Beef Samples from Aksaray Province, Türkiye

Hatice Berna Poçan; Özge Oktay Ayan; Burçak Aslan Çelik; Özgür Yaşar Çelik; Muhammed Yasul; Adnan Ayan; Ali Bilgin Yılmaz; İlker Çamkerten; Mustafa Ardıç

doi:10.24925/turjaf.v14i6.1488-1493.8419

Authors

Hatice Berna Poçan Department of Food Processing, Çumra Vocational School, Selçuk University, Konya, Türkiye https://orcid.org/0000-0002-2094-3685
Özge Oktay Ayan Department of Parasitology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Türkiye https://orcid.org/0000-0003-2577-3774
Burçak Aslan Çelik Department of Parasitology, Faculty of Veterinary Medicine, Siirt University, Siirt, Türkiye https://orcid.org/0000-0002-0130-970X
Özgür Yaşar Çelik Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, Türkiye https://orcid.org/0000-0001-6365-2688
Muhammed Yasul Department of Parasitology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Türkiye https://orcid.org/0000-0001-7948-5773
Adnan Ayan Department of Parasitology, Faculty of Veterinary Medicine, Aksaray University, Aksaray, Türkiye https://orcid.org/0009-0009-6335-7019
Ali Bilgin Yılmaz Faculty of Health, Van Yuzuncu Yil University, Van, Türkiye https://orcid.org/0000-0003-0749-2418
İlker Çamkerten Department of Internal Medicine, Faculty of Veterinary Medicine, Aksaray University, Aksaray, Turkiye https://orcid.org/0000-0002-6952-7703
Mustafa Ardıç Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Aksaray University, Aksaray, Turkiye https://orcid.org/0000-0002-4193-1990

DOI:

https://doi.org/10.24925/turjaf.v14i6.1488-1493.8419

Keywords:

Toxoplasma gondii, minced beef, PCR, Aksaray, Phylogenetic analysis

Abstract

Toxoplasma gondii is an apicomplexan protozoan commonly found in both humans and animals worldwide. This study aimed to investigate the presence of T. gondii in minced beef samples collected from butcher shops and supermarkets in Aksaray Province, Türkiye. A total of 100 samples were analyzed by PCR, of which 3 (3%) tested positive. The prevalence was 4% in butcher samples and 2.67% in supermarket samples. Sequencing analysis of three isolates revealed high genetic similarity to previously reported T. gondii strains deposited in GenBank. This study provides the first molecular evidence of T. gondii in minced beef from Aksaray, highlighting potential public health concerns regarding foodborne transmission.

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Molecular Detection and Phylogenetic Analysis of Toxoplasma gondii in Minced Beef Samples from Aksaray Province, Türkiye

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