Cross-Species Transferability of Cotton SSR Markers within Malvaceae
DOI:
https://doi.org/10.24925/turjaf.v13is2.3591-3597.8210Keywords:
Genimic SSRs, EST-SSRs, genomic data, vital crops, microsatellitesAbstract
Conserved nature of genomes can be used advantageously to study the landscape of allelic diversity in understudied species of the same genus or family. In the present studies, a total of 54 SSRs regions including both EST and genomic belonging to G. raimondii, G. barbadense and G. hirsutum were selected from the available database for studying the cross-species transferability to 12 understudied species of the family Malvaceae and G. hirsutum (upland cotton). In total 24 SSRs (44.44%) including 5 gSSRs and 19 EST-SSRs showed amplification in the species used in the study depicting a higher amplification ratio of EST-SSRS as compared to genomic SSRs. BNL4037 and BNL3089 both have shown maximum transferability (69.23 %) followed by HAU1980 (61.5%) among all markers tested, while NAU2679, NAU3206, NAU3538, NAU3793, NAU3903, NAU5423 and BNL150 shown 7.69% transferability. In all 13 species allele amplification ranged from 1 to 12 and maximum number of alleles (12) were amplified by primer BNL 3089. Among the species used in study highest transferability rate (100 %) was observed in G. hirsutum followed by Malvaviscus arboreus (45.83 %). However, none of the primer amplified SSR regions in Grewia asiatica and Malva parviflora. The results of this study demonstrated a notable SSR region transferability from cotton to other members of Malvaceae. These findings can contribute to the development of an SSR marker pool, which may aid genetic analysis as well as the allelic diversity assessment of species within the Malvaceae that possess less available genomic information.
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