The Effects of Adding Styrax Officinalis L. Leaves on The Fermentation Quality, Nutrient Composition and Microbiological Characteristics of Corn Silage
DOI:
https://doi.org/10.24925/turjaf.v13i11.3369-3375.8162Keywords:
Styrax officinalis L.,, Corn silage,, Fermentation quality, Silage additive , Microbial compositionAbstract
In the study, fresh corn harvested during the milk stage and cut into pieces approximately 1,5-2,0 cm in size was mixed with SL from different vegetation stages (pre-flowering, flowering, seed setting, and fruiting) at levels of 0 (SL0), 1% (SL1), and 2% (SL2) levels. After the additives were added, approximately 500 g of the sample was placed in plastic bags, compressed, and vacuumed to remove the air inside. A total of 45 packages, with 5 replicates for each group, were left to ferment under laboratory conditions for 24 months. After fermentation, chemical, microbiological, and sensory analyses were performed on the opened silage samples.The addition of Styrax officinalis leaves to corn silage significantly improved the chemical composition, fermentation characteristics, and microbial composition of the silage. The content of crude protein (CP) was determined to be highest in silage samples prepared by treatment with SL2 applied to material harvested before flowering (16,04%), and the treatments significantly reduced the ratio of ammonia nitrogen to total nitrogen (NH₃-N/TN) compared to the SL0 group (P < 0,05). In the additive groups, the lactic acid bacteria (LAB) population increased, while the yeast population decreased. In particular, additives silages prepared from corn materials harvested before flowering and during the fruiting period showed more successful fermentation results with low pH values, high protein levels, and lower dry matter loss (DML) compared to the control group. The highest sensory evaluation scores were obtained in the silage where additions were made during the flowering period.In conclusion, the addition of SL to corn material at the SL1 level, especially during the pre-flowering and fruiting periods, improves fermentation quality, enhances LAB development, limits yeast counts, and increases feed values. In this context, it can be concluded that the SL has the potential to be an alternative source of natural, economical, and sustainable silage additive.
References
Akbağ, H. I., Akgül, C., & Ataşoğlu, C. (2024). Antibacterial activity of some shrub leaves consumed by goats. Kadirli Uygulamalı Bilimler Fakültesi Dergisi, vol.4, no.2, 386-397.
Akyıldız, R. (1984). Besin analizleri. Ankara Üniversitesi Ziraat Fakültesi Yayınları No: 868.
Anonim. (1986). The analysis of agricultural materials (3rd ed.). London: Ministry of Agriculture, Fisheries and Food, Reference Book, 427–428.
Balyen, İ., & Kiraz, A. B. (2024). Determination of nutritional value and methane production potential of Styrax tree (Styrax officinalis L.) leaves. Heliyon, 10(14), e27306. https://doi.org/10.1016/j.heliyon.2024.e27306
Chen, L., Weinberg, Z. G., & Chen, Y. (1994). The effect of lactic acid bacteria inoculants on the fermentation and aerobic stability of wheat and corn silages. Journal of Industrial Microbiology, 13(3), 208–212.
Davis, P. H. (1972). Flora of Turkey and the East Aegean Islands (Vol. 4). Edinburgh: University of Edinburgh Press.
DLG, (1987). Pattern for the evulation of the fermentation quality of grass silages on the basis of chemical analyses. Frankfurt am Main: Deutsche Landwirtschafts Gesellschaft. Bewertungvon Grünfutter, Silageund Heu. Merkblatt, No.224 DLG Verlag, Frankfurt
Dökülgen, H., & Temel, S. (2019). Menengiç ve tesbih çalısının mevsimlere ve otlanan bitki kısımlarına göre yem kalitesinin belirlenmesi. Harran Tarım ve Gıda Bilimleri Dergisi, 23(2), 178–188.
Ertekin, İ., Atış, İ., Yılmaz, Ş., Can, E., & Kızılşimşek, M. (2019). Comparison of shrub leaves in terms of chemical composition and nutritive value. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 22(5), 781–786. https://doi.org/10.18016/ksutarimdoga.vi.549952
Erten, K., Kaya, A., & Koç, F. (2022). Bakteriyel inokulant ve organik asit ilavesi ile yeniden silolamanın mısır silajının aerobik stabilitesi ve in vitro gaz üretim parametreleri üzerine olan etkileri. Journal of the Institute of Science and Technology, 12(4), 2568–2580. https://doi.org/10.21597/jist.1137972.
Esen, S., Koc, F., Özdüven, L., Eseceli, H., Cabi. E., & Karadağ, H. (2022). In situ and in vitro nutritive value assessment of styrax officinalis L. as an alternative forage source for goat feeding. Journal of Agricultural Sciences, 28(2), 181-188.
Genç, L., & Soysal, A. (2018). Deneme ve araştırma yöntemleri. Ankara: Ankara Üniversitesi Yayınları.
He, L., Zhou, W., Wang, C., Yang, F., Chen, X., & Zhang, Q. (2019). Effect of cellulase and Lactobacillus casei on ensiling characteristics, chemical composition, antioxidant activity, and digestibility of mulberry leaf silage. Journal of Dairy Science, 102(11), 9919–9931. https://doi.org/10.3168/jds.2019-16468
Khorvash, M., Ghorbani, G. R., Kazemi-Bonchenari, M., Ghaffari, M. H., & Robinson, P. H. (2006). Influence of ensiling characteristics on aerobic stability of sugar beet pulp silage. Animal Feed Science and Technology, 126(3–4), 161–168. https://doi.org/10.1016/j.anifeedsci.2005.07.009
Koç, F., & Coşkuntuna, L. (2003). Silo Yemlerinde Organik Asit Belirlemede İki Farklı Metodun Karşılaştırılması. Hayvansal Üretim, 44 (2): 37-47
Koç, F., Özdüven, M. L., Demirci, A. Ş., & Şamlı, H. E. (2018). Mısır silajlarında saha şartlarında aerobik stabilite süresince mikrobiyal kompozisyondaki değişikliklerin termal kamera görüntüleme tekniği ile değerlendirilmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 21(2), 167–174.
McDonald, P., Edwards, R. A., Greenhalgh, J. F. D., & Morgan, C. A. (2002). Animal nutrition (6th ed.). Harlow, UK: Pearson Education.
McDonald, P., Henderson, A.R., & Heron, S.J.E. 1991. The Biochemistry of Silage. Second Edition. Chalcombe Publication, Marlow, England. 340 p.
Mendieta-Araica, B., Spörndly, R., Reyes-Sánchez, N., & Spörndly, E. (2009). Silage quality when Moringa oleifera is ensiled in mixtures with elephant grass, sugar cane and molasses. Grass and Forage Science, 64(4), 364–371. https://doi.org/10.1111/j.1365-2494.2009.00696.x
Okumuş, A. (2021). İkinci ürün mısır silajına fındık zurufu ilavesinin silaj fermantasyonu, aerobik stabilite ve in vitro gaz üretimi üzerine etkileri. Yüksek lisans tezi, Bursa Uludağ Üniversitesi.
Parlar, T., & Koç, F. (2020). Toplam rasyon karışımı kullanılan bir süt sığırı işletmesinin besleme açısından değerlendirilmesi. Erciyes Tarım ve Hayvan Bilimleri Dergisi, 3(1), 24–32.
Paydaş, E., Savrunlu, Z., Savrunlu M., & Denek, N. (2019). Mısır silajına farklı oranlarda Antep fıstığı (pistacia vera l.) dış kabuğu ilavesinin silaj kalitesi ve in vitro metan gazı oluşumu üzerine etkisinin araştırılması. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 16(1), 16-22. https://doi.org/10.32707/ercivet.538026.
Seale, D. R., Pahlow, G., & Spoelstra, S. F. (1990). The significance of aerobic spoilage of silage. Institute of Grassland and Environmental Research Report, 17–32.
Şimşek, N., & Kamalak, A. (2019). Bazı ağaç yapraklarının antimetanojenik özelliklerinin in vitro gaz üretim tekniği ile belirlenmesi. Black Sea Journal of Agriculture, 2(1), 1–5.
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583–3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2.
Wang, Y., Zhang, Y., Li, J., Lin, J., Zhang, N., & Cao, W. (2021). Biogas energy generated from livestock manure in China: Current situation and future trends. Journal of Environmental Management, 297, 113324. https://doi.org/10.1016/j.jenvman.2021.113324.
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