Physicochemical and Phytochemical Properties of Tokat Pepper Fruit Flesh and Seeds

Authors

DOI:

https://doi.org/10.24925/turjaf.v13i10.3032-3039.8088

Keywords:

Tokat pepper, three-lobed, phytochemical properties, Phytochemical Properties , Antioxidant

Abstract

In this study, it was aimed to determine the physicochemical and phytochemical properties of the edible part of Tokat pepper, locally known as “üç burun” (three-lobed) and widely cultivated in the Tokat region as an important traditional and commercial agricultural product, as well as the seeds released during the processing of this edible part. The L* value of Tokat pepper was determined as 44.62 on average, while the a* value was -20.59 (green tone), and the b* value was 34.34 (dominant yellow tone). The protein (4.11%) and ash (1.02%) contents of the seed part were found to be higher than those of the fruit flesh (2.01% and 0.86%, respectively). Total phenolic content, flavonoid content, and antioxidant capacity (by FRAP and ABTS methods) were analyzed in both the fruit flesh and seeds. The total phenolic content was determined as 171.46 mg GAE/100 g in the fruit flesh and 124.08 mg GAE/100 g in the seeds. Total flavonoid content was measured as 138.50 mg QE/100 g in the fruit and 123.29 mg QE/100 g in the seeds. In the antioxidant capacity analyses, the ABTS value was higher in the seeds (250.09 mg TE/100 g) than in the fruit flesh (184.94 mg TE/100 g), whereas the FRAP value was higher in the fruit flesh (131.69 mg TE/100 g) than in the seeds (72.46 mg TE/100 g). The findings indicate that both the edible part and the seeds, which are often considered waste, of Tokat pepper are rich in biochemical compounds and possess potential as functional food ingredients.

References

Ab Rahman, M. H., Jusoh, Y. M. M., & Hashim, Z. (2024). Total phenolic, total flavonoid and antioxidant activity of Capsicum annuum seed and pericarp extracts. Journal of Bioprocessing and Biomass Technology, 3(2), 107–111. https://doi.org/10.11113/bioprocessing.v3n2.62

AOAC. (2005). Official methods of analysis. Association of Official Analytical Chemists.

Asrat, U., Minuye, M., & Woldegiorgis, E. (2025). Physicochemical properties of pepper cultivars (Capsicum annuum L.) grown at different locations in Ethiopia. International Journal of Vegetable Science, 31(3), 352–375. https://doi.org/10.1080/19315260.2025.2475827

Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1), 70–76. https://doi.org/10.1006/abio.1996.0292

Borges-Machado, A. L., Ribeiro-Sanches, M. A., Martins, M. J. N., Luna-Flores, M., Luna-Solano, G., de Souza, S. J. F., & Telis-Romero, J. (2024). Drying kinetics and water adsorption properties of jalapeño pepper seeds (Capsicum annuum L.): A study aiming at their recovery and integral exploitation. LWT, 204, 116452. https://doi.org/10.1016/j.lwt.2024.116452

Cemeroğlu, B. (2010). Gıda analizleri (34. baskı). Gıda Teknolojisi Derneği Yayınları.

Çaylak, S. (2018). Farklı biber anaçları üzerine aşılı sivri ve dolma biberlerde, verim ve kalite özelliklerinin belirlenmesi. [Yüksek lisans tezi, Akdeniz Üniversitesi].

Çiftci, H. N., Gundogdu, M. A., & Kaynas, K. (2023). Farklı rakımlarda yetiştirilmiş Yenice kırmızı biberinin kalite değişimleri. International Journal of Innovative Approaches in Science Research, 7(2), 47-61. https://doi.org/10.29329/ijiasr.2023.578.1

Dağhan, Ş., & Vardin, H. (2019). Şanlıurfa bibertohumu yağının yağ asitleri kompozisyonu ve mineral içeriğinin belirlenmesi. Harran Üniversitesi Mühendislik Dergisi, 4(3), 49–57.

De Almeida, B. M., & Clarindo, W. R. (2025). A multidisciplinary and integrative review of the structural genome and epigenome of Capsicum L. species. Planta, 261(4), 82. https://doi.org/10.1007/s00425-025-04653-w

Denev, P., Todorova, V., Ognyanov, M., Georgiev, Y., Yanakieva, I., Tringovska, I., … Kostova, D. (2019). Phytochemical composition and antioxidant activity of 63 Balkan pepper (Capsicum annuum L.) accessions. Journal of Food Measurement and Characterization, 13, 2510–2520. https://doi.org/10.1007/s11694-019-00171-y

Dısko Younus, S., Yanar, D., & Atakan, E. (2023). Thrips (Insecta: Thysanoptera) species in pepper fields in Tokat Province, Türkiye. Journal of Agricultural Faculty of Gaziosmanpaşa University, 40(2), 89–95. https://doi.org/10.55507/gopzfd.1330038

FAOSTAT. (2023). FAO statistical database. https://www.fao.org/faostat/en/#data/QC

Francis, F. J. (1995). Quality as influenced by color. Food Quality and Preference, 6(3), 149–155.

Hamed, M., Kalita, D., Bartolo, M. E., & Jayanty, S. S. (2019). Capsaicinoids, polyphenols and antioxidant activities of Capsicum annuum: Comparative study of the effect of ripening stage and cooking methods. Antioxidants, 8(9), 364.

Hayouni, E. A., Abedrabba, M., Bouix, M., & Hamdi, M. (2007). The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food chemistry, 105(3), 1126-1134. https://doi.org/10.1016/j.foodchem.2007.02.010

Howard, L. R., Talcott, S. T., Brenes, C. H., & Villalon, B. (2000). Changes in phytochemical and antioxidant activity of selected pepper cultivars (Capsicum species) as influenced by maturity. Journal of Agricultural and Food Chemistry, 48(5), 1713–1720.

Islam, M., Satyaranjan, S. A. H. A., Akand, H., & Rahim, A. (2011). Effect of spacing on the growth and yield of sweet pepper (Capsicum annuum L.). Journal of Central European Agriculture. 12(2), p.328-335. https://doi.org/10.5513/jcea.v12i2.970

Kartal, G., Kartal, H., & Gebeloğlu, N. (2024). Bazı yerelbiber tiplerinden elde edilen melezlerin morfolojik özelliklerinin ve Domates Lekeli Solgunluk Virüsüne (TSWV) dayanıklılığının belirlenmesi. Harran Tarım ve Gıda Bilimleri Dergisi, 28(3), 508–523. https://doi.org/10.29050/harranziraat.1434593

Kavuncuoğlu, H., Capar, T. D., & Yalçın, H. (2021). Total phenolic contents, antioxidant activities and antioxidant capacities of some selected pepper (Capsicum annuum L.) pulps and seeds. Avrupa Bilim ve Teknoloji Dergisi, (21), 581–586. https://doi.org/10.31590/ejosat.793148

Kaya, C., Güldane, M., Topuz, S., & Bayram, M. (2018). Determination of some properties of Turkish delight produced by the addition of pomegranate juice. Turkish Journal of Agriculture-Food Science and Technology, 6(12), 1814–1819.

Kebu, Z., Gure, A., & Molole, G. J. (2024). Total phenolic and flavonoid content, lipophilic components, and antioxidant activities of Capsicum annuum varieties grown in Omo Nada, Jimma, Ethiopia. Natural Product Communications, 19(12), Article 1934578X241306244. https://doi.org/10.1177/1934578X241306244

Keleş, D., Rastgeldi, U., Karipçin, Z., Karagül, S., Soylu, M. K., Çömlekçioğlu, N., & Büyükalaca, S. (2016). Seleksiyon yoluyla Şanlıurfa biber ıslahı. Alatarım, 15(1), 39–44.

Liu, Y., Chen, Y., Wang, Y., Chen, J., Huang, Y., Yan, Y., … Xiao, Y. (2020). Total phenolics, capsaicinoids, antioxidant activity, and α-glucosidase inhibitory activity of three varieties of pepper seeds. International Journal of Food Properties, 23(1), 1016–1035. https://doi.org/10.1080/10942912.2020.1775646

Maurya, V. K., Gothandam, K. M., Ranjan, V., Shakya, A., & Pareek, S. (2018). Effect of drying methods (microwave vacuum, freeze, hot air and sun drying) on physical, chemical and nutritional attributes of five pepper (Capsicum annuum var. annuum) cultivars. Journal of the Science of Food and Agriculture, 98(9), 3492–3500. https://doi.org/10.1002/jsfa.8868

Medina-Juárez, L. Á., Molina-Quijada, D. M., Sánchez, C. L. D. T., González-Aguilar, G. A., & Gámez-Meza, N. (2012). Antioxidant activity of peppers (Capsicum annuum L.) extracts and characterization of their phenolic constituents. Interciencia, 37(8), 588–593.

Oli, P., Rawat, P., Punetha, S., & Shukla, S. (2025). Antioxidant activities and biochemical analysis of Capsicum annuum L. varieties at different ripening stages. Current Science, 128(10). https://doi.org/10.18520/cs/v128/i10/999-1005

Palma, J. M., Terán, F., Contreras-Ruiz, A., Rodríguez-Ruiz, M., & Corpas, F. J. (2020). Antioxidant profile of pepper (Capsicum annuum L.) fruits containing diverse levels of capsaicinoids. Antioxidants, 9(9), 878. https://doi.org/10.3390/antiox9090878

Papathanasiou, T., Gougoulias, N., Karayannis, V. G., & Kamvoukou, C. A. (2021). Investigation of the total phenolic content and antioxidant capacity of three sweet pepper cultivars (Capsicum annuum L.) at different development and maturation stages. Periodica Polytechnica Chemical Engineering, 65(2), 219–228. https://doi.org/10.3311/PPch.15553

Putnik, P., Barba, F. J., Španić, I., Zorić, Z., Dragović-Uzelac, V., & Kovačević, D. B. (2017). Green extraction approach for the recovery of polyphenols from Croatian olive leaves (Olea europea). Food and Bioproducts Processing, 106, 19–28.

Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9–10), 1231–1237. https://doi.org/10.1016/S0891-5849(98)00315-3

Soare, R., Dinu, M., Băbeanu, C., Popescu, M., & Popescu, A. (2017). Nutritional value and antioxidant activities in fruit of some cultivars of pepper (Capsicum annuum L.). Journal of Agroalimentary Processes and Technologies, 23(4), 217–222.

Sora, G. T. S., Haminiuk, C. W. I., da Silva, M. V., Zielinski, A. A. F., Gonçalves, G. A., Bracht, A., & Peralta, R. M. (2015). A comparative study of the capsaicinoid and phenolic contents and in vitro antioxidant activities of the peppers of the genus Capsicum: An application of chemometrics. Journal of Food Science and Technology, 52, 8086–8094. https://doi.org/10.1007/s13197-015-1935-8

Tanrıkulu, E. (2024). Bursa Yenişehir çevresi sivri biber (Capsicum annuum L.) çeşitlerinin organik tarım koşullarında verim ve kalite özelliklerinin değerlendirilmesi [Yüksek lisans tezi, Bursa Uludağ Üniversitesi].

Yang, Y., Gao, C., Ye, Q., Liu, C., Wan, H., Ruan, M., … Cheng, Y. (2024). The influence of different factors on the metabolism of capsaicinoids in pepper (Capsicum annuum L.). Plants, 13(20), 2887. https://doi.org/10.3390/plants13202887

Zhu, H., Wang, Y., Liu, Y., Xia, Y., & Tang, T. (2010). Analysis of flavonoits in Portulaca oleracea L. by UV–vis spectrophotometry with comparative study on different extraction technologies. Food Analytical Methods, 3(2), 90–97.

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Published

13.10.2025

How to Cite

Esin, E., Topuz Türker, S., Kaya, C., & Bayram, M. (2025). Physicochemical and Phytochemical Properties of Tokat Pepper Fruit Flesh and Seeds. Turkish Journal of Agriculture - Food Science and Technology, 13(10), 3032–3039. https://doi.org/10.24925/turjaf.v13i10.3032-3039.8088

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Research Paper