In vitro and Ruminal Characteristics of the Three Selected Nigerian Herbs: Phyllanthus amarus, Ocimum gratissimum and Lactuca taraxacifolia as Feed Additives in Ruminant Production
DOI:
https://doi.org/10.24925/turjaf.v14i1.1-8.8012Keywords:
Herbal plants, feed additives, Ruminants, In vitro fermentation, Microbial populationAbstract
This study evaluated the effects of selected herbal feed additives on in vitro fermentation and ruminal characteristics of West African Dwarf (WAD) goats. Twenty goats (4 per treatment) were assigned to five dietary treatments in a 12-week completely randomised design: basal diet only (T1, control), diet + Ocimum gratissimum leaf meal (T2), diet + Phyllanthus amarus leaf meal (T3), diet + Lactuca taraxacifolia leaf meal (T4), and diet + a mixture of the three herbs (T5). Phytochemical screening confirmed the presence of saponins, tannins, and alkaloids in the herbs. The additives, incorporated at 3 g/kg feed, were offered at 5% of body weight. Feed samples were incubated for 24 h, with gas volume measured every 3 h, and methane subsequently estimated. Gas data were used to derive metabolizable energy (ME), organic matter digestibility (OMD), and short-chain fatty acid (SCFA) concentrations. At the end of the feeding trial, the rumen liquor was analysed for its fermentation characteristics and microbial populations. The herbs were rich in crude protein (CP) and moderate in carbohydrate content. The mixture diet (T5) contained the highest CP, while T2 had the greatest crude fibre. In vitro fermentation showed increasing gas production with incubation time, with T1 and T4 yielding the highest gas volumes. Methane production was lower in T3 and T5 compared with other treatments. Similarly, T1 and T4 recorded the highest ME, OMD, and SCFA values. However, T3 and T5 markedly improved ruminal fermentation and beneficially modified microbial populations. In conclusion, P. amarus (T3) and the combined herbal treatment (T5) demonstrated potential as natural feed additives for mitigating methane emissions and enhancing rumen ecology, thereby improving ruminant productivity.
References
Abd El Tawab, A. M., Khattab, M. S. A., Hadhoud, F. I., & Shaaban, M. M. (2021). Effect of mixture of herbal plants on ruminal fermentation, degradability and gas production. Acta Scientiarum. Animal Sciences, 43, 1–8. https://doi.org/10.4025/actascianimsci.v43i1.51241
Abdel-Hamid, N. M., Nazmy, M. H., Mahmoud, A. W., & Fawzy, M. A., Youssof, M. (2011). A survey on herbal management of hepatocellular carcinoma. World Journal of Hepatology, 3(7), 175–183. https://doi.org/10.4254/wjh.v3.i7.175
Abdullah, M. A. M., & Farghaly, M. M. (2019). Impact of partial replacement of cotton seed meal by Nigella sativa meal on nutrients digestibility, rumen fermentation, blood parameters, growth performance of growing lambs. Egyptian Journal of Nutrition and Feeds, 22(2), 11–20.
Aberu, A., Carulla, J. E., Lascano, C. E., Diaz, T. E., Kreuzer, N., & Hess, H. D. (2004). Effect of fruits on ruminal fermentation and duodenal nitrogen flow of sheep fed a tropical grass diet with and without legine. Journal of Animal Science, 82, 1392–1399. https://doi.org/10.2527/2004.8251392x
Adinortey, M. B., Sarfo, J. K., Quayson, E. T., Weremfo, A., Adinortey, C. A., & Ekloh, W. (2012). Phytochemical screening, proximate and mineral composition of Launaea taraxacifolia leaves. Research Journal of Medicinal Plants, 6(2), 171–179. https://doi.org/10.3923/rjmp.2012.171.179
Akinfemi, A., & Ladipo, M. K. (2014). Nutritional potential of some selected weed species in north central Nigeria using in vitro gas production technique. International Journal of Agricultural Science, 4(4), 214–217.
Amany, A. K., El-Badawy, M. M., & Hanaa, S. S. (2022). Effect of some medical herbs on productive performance of lactating Zaraibi goats. Egyptian Journal of Nutrition and Feeds, 25(3), 303–321.
Anantasook, N., & Wanapat, M. (2012). Influence of rain tree pod meal supplementation on rice straw based diets using in vitro gas fermentation technique. Asian-Australasian Journal of Animal Sciences, 25(3), 325–331. https://doi.org/10.5713/ajas.2011.11362
AOAC. (2012). Official methods of analysis (19th ed.). Association of Official Analytical Chemists.
Arawande, J. O., Amoo, I., & Lajide, L. (2013). Chemical and phytochemical composition of wild lettuce (Launaea taraxacifolia). Journal of Applied Phytotechnology in Environmental Sanitation, 2(1), 25-30.
Arhab, R., Karima, K., Nassima, L., Hakim, B., Besma, H., & Hacene, B. (2013). Effect of essential oils extracted from Satureja calamintha, Mentha pulegium and Juniperus phoenicea on in vitro methanogenesis and fermentation traits of vetch oat hay. African Journal of Environmental Science and Technology, 7, 140–144. https://doi.org/10.5897/AJEST12.191
Bello, O. M., Abiodun, O. B., & Oguntoye, S. O. (2018). Insight into the ethnopharmacology, phytochemistry, pharmacology of Launaea taraxacifolia (Willd) Amin ex C. Jeffrey as an underutilized vegetable from Nigeria: a review. Annals of the University Dunarea de Jos of Galati Fascicle VI: Food Technology/Analele Universităţii Dunărea de Jos din Galaţi, Fascicula VI: Tehnologia Produselor Alimentare, 42(2).
Busquet, M., Calsamiglia, S., Ferret, A., Cardozo, P. W., & Kamel, C. (2005). Effects of cinnamaldehyde and garlic oil on rumen microbial fermentation in a dual flow continuous culture. Journal of Dairy Science, 88(7), 2508–2516. https://doi.org/10.3168/jds.S0022-0302(05)72928-3
Cao, Y. N., Sun, Y. R., Tang, Y. M., Xie, X. L., Li, Q. Y., Mou, F. Z., & Sun, H. X. (2024). Effect of high oxalic acid intake on growth performance and digestion, blood parameters, rumen fermentation and microbial community in sheep. Small Ruminant Research, 237, 107324.
Chaudhry, A. S., & Khan, M. M. H. (2012). Impacts of different spices on in vitro rumen dry matter disappearance, fermentation and methane of wheat or ryegrass hay based substrates. Livestock Science, 146(1), 84–90. https://doi.org/10.1016/j.livsci.2012.02.001
Dansi, A., Vodouhè, R., Azokpota, P., Yedomonhan, H., Assogba, P., Adjatin, A., ... & Akpagana, K. J. T. S. W. J. (2012). Diversity of the neglected and underutilized crop species of importance in Benin. The scientific world journal, 2012(1), 932947.
Dong, G., Wang, X., Liu, Z., & Wang, F. (2010). Effects of phytogenic products on in vitro rumen fermentation and methane emission in goats. Journal of Animal and Feed Sciences, 19(2), 218–219.
Edeoga, H. O., Omosun, G., & Uche, L. C. (2006). Chemical composition of Hyptis suaveolens and Ocimum gratissimum hybrids from Nigeria. African journal of Biotechnology, 5(10).
Fievez, V., Babayemi, O. J., & Demeyer, D. (2005). Estimation of direct and indirect gas production in syringes: A tool to estimate short chain fatty acid production requiring minimal laboratory facilities. Animal Feed Science and Technology, 124(1–2), 197–210.
Filípek, J., & Dvořák, R. (2009). Determination of the volatile fatty acid content in the rumen liquid: Comparison of gas chromatography and capillary isotachophoresis. Acta Veterinaria Brno, 78(4), 627–633.
Galyean, M. (1989). Laboratory Procedure in Animal Nutrition Research. Department of Animal and Life Science, New Mexico State University, LasCruces. 107–122pp
Gideon, O. O., Mahboob, A. J., Olusanya, A. O., Abdulfatai, B. R., & Adepeju, O. O. (2018). Proximate analysis, mineral composition, and antioxidant properties of bitter leaf and scent leaf. International Journal of Vegetable Science, 24(1), 1–9.
Goodland, R., & Anhang, J. (2009). Livestock and climate change: What if the key actors in climate change are cows, pigs, and chickens? World Watch, 22(6), 10–19.
Kim, E. T., Kim, C. H., Min, K. S., & Lee, S. S. (2012). Effects of plant extracts on microbial population, methane emission and ruminal fermentation characteristics in vitro. Asian-Australasian Journal of Animal Sciences, 25(6), 806–811.
Ladipo, M. K., Doherty, V. F., & Kanife, U. C. (2010). Phytochemical screening and antibacterial investigation of the extract of Ocimum gratissimum (scent leaf) on selected Enterobacteriaceae. Journal of Clinical Pathology, 6(2), 75–84.
Lila, Z. A., Mohammed, N., Kanda, S., Kamada, T., & Itabashi, H. (2003). Effect of sarsaponin on ruminal fermentation with particular reference to methane production in vitro. Journal of Dairy Science, 86(10), 3330–3336.
Mariam, A. G., El-Zarkouny, S. Z., El-Shazly, K. A., & Sallam, S. M. A. (2014). Impact of essential oils blend on methane emission, rumen fermentation characteristics and nutrient digestibility in Barki sheep. Journal of Agricultural Science, 6(3), 144–152.
Mgbeje, B. I. A., Umoh, E. U., & Emmanuel-Ikpeme, C. (2019). Comparative analysis of phytochemical composition of four selected tropical medicinal plants namely: Ocimum gratissimum, Piper guineense, Gongronema latifolium and Vernonia amygdalina. Journal of Complementary and Alternative Medical Research, 7(3), 1–11.
Mohammad, E. N. S., & Moeini, M. M. (2015). The influence of ginger (Zingiber officinale) on in vitro rumen fermentation patterns. Annual Research & Review in Biology, 5(1), 54–63.
Namrata, J., Saritha, R. K., Datta, D., Singh, M., Dubey, R. S., Rai, A. B., & Rai, M. (2010). Molecular characterization of tomato leaf curl Palampur virus and pepper leaf curl betasatellite naturally infecting pumpkin (Cucurbita moschata) in India. Indian Journal of Virology, 21, 128-132.
Norton, B. W. (2003). The nutritive value of tree legumes. In R. G. Gutteridge & H. M. Shelton (Eds.), Forage tree legumes in tropical agriculture. FAO. http://www.fao.org/ag/AGP/AGPC/doc/Publicat/Guttshel/x5556e0j.htm#4.1
Nte, I. J., Oleforuh-Okoleh, V. U., & Nwiidae, B. S. (2016). Effect of scent leaf (Ocimum gratissimum) administration on growth performance and carcass traits of broiler chicks. In Nigeria Society of Animal Science Production (NSAP) Conference FUNAAB Abeokuta, NSAP.(Ed.)., Nigeria Society of Animal Science Production, Nigeria.
Odhaib, K. J., Adeyemi, K. D., Ahmed, M. A., Jahromi, M. F., Jusoh, S., Samsudin, A. A., & Sazili, A. Q. (2018). Influence of Nigella sativa seeds, Rosmarinus officinalis leaves and their combination on growth performance, immune response and rumen metabolism in Dorper lambs. Tropical Animal Health and Production, 50(5), 1011–1023.
Ogunbosoye, D. O., Tona, G. O., & Otukoya, F. K. (2015). Evaluation of the nutritive value of selected browse plant species in the Southern Guinea Savannah of Nigeria for feeding to ruminant animals. British Journal of Applied Science & Technology, 7(4), 386–395.
Redoy, M. R. A., Shuvo, A. A. S., Cheng, L., & Al-Mamun, M. (2020). Effect of herbal supplementation on growth, immunity, rumen histology, serum antioxidants and meat quality of sheep. Animals, 14(11), 2433–2441.
Rezaei, N., & Pour, H. A. (2012). Evaluation effect of thyme extract on degradability of soybean meal with gas production technique. Advances in Bioresearch, 3(1), 45–47.
Rosine, B. T., Fidèle, N., & David, M. M. (2022). Pharmacological potential of Lactura taraxacifolia leaf on blood glucose levels, lipid profile, and oxidative stress parameters in diabetic rats. Journal of Pharmacology and Biological Science, 10(1), 29–36.
Samuel, M., Sagathewan, S., Thomas, J., & Mathen, G. (1997). An HPLC method for estimation of volatile fatty acids of ruminal fluid. Indian Journal of Animal Science, 67(9), 805–811.
Sirohi, S. K., Pandey, N., Goel, N., Singh, B., Mohini, M., Pandey, P., & Chaudhry, P. P. (2009). Microbial activity and ruminal methanogenesis as affected by plant secondary metabolites in different plant extracts. International Journal of Civil and Environmental Engineering, 1(1), 1–8.
Tag El-Din, A. E., Moharam, M. S., Nour, A. A., & Nasser, M. E. (2012). Effect of some herbs on the rumen fermentation: I. Effect of ginger (Zingiber officinale) and garlic (Allium sativum) on gas production, energy values, organic matter digestibility and methane emission, in vitro. Journal of Agricultural and Environmental Science, 11(1), 33–53.
Van Soest, P. J. (1994). Nutritional ecology of the ruminant (2nd ed.). Cornell University Press.
Verma, S., Sharma, H., & Garg, M. (2014). Phyllanthus amarus: A review. Journal of pharmacognosy and Phytochemistry, 3(2), 18-22.
Wanapat, M., Kang, S., Khejornsart, P., & Wanapat, S. (2013). Effects of plant herb combination supplementation on rumen fermentation and nutrient digestibility in beef cattle. Asian-Australasian Journal of Animal Science, 26(8), 1127–1136.
Wang, M., Wu, L., Guo, Y., Sun, J., Deng, M., Liu, G., Li, Y., & Sun, B. (2023). Effects of fermented herbal tea residue on meat quality, rumen fermentation parameters and microbes of black goats. AMB Express, 13(1), 106.
Winifred, U., & Alexander, M. (2018). Biochemical studies of the ameliorating effects of bitter leaf and scent leaf extracts on diabetes mellitus in humans. IJCCP, 4(1), 29-46.
Yusuf, A. L. (2015). Evaluation of dietary supplementation of Andrographis paniculata on growth performance and meat quality of Boer goats (Ph.D. thesis). Universiti Putra Malaysia. http://www.lib.upm.edu.my
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