The Determination of Some Phytochemical Compounds and Antiradical Activities of Urtica dioica L., Morus nigra L., and Glycyrrhiza glabra L. Powder as Feed Additives
DOI:
https://doi.org/10.24925/turjaf.v13i11.3230-3235.7924Keywords:
Black mulberry, licorice root, nettle, 'antiradical', fenolic, flavonoidAbstract
This study was assessed the approximate their antioxidant capacity and certain bioactive components of Glycyrrhiza glabra L., Morus nigra L. and Urtica dioica L. which can be employed as feed supplements. DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging tests were carried out to measure the antiradical properties of these plants. The average DPPH radical cleaning activities of Morus nigra L., Glycyrrhiza glabra L. and Urtica dioica L.’s powder extracts of methanol, were measured as 86.37%, 92.90%, 71.98% respectively. The average ABTS radical cleaning activities of Morus nigra L., Glycyrrhiza glabra L. and Urtica dioica L.’s powder extracts of methanol were measured as 90.83%, 98.07%, 83.62% respectively. Morus nigra L. was found to contain the highest levels of flavonoid (19.80 μg GAE/g) and phenolic (40.79 μg GAE/g) components. It has been measured those phenolic acids and flavonoid were found in different proportions in these plants. While the highest concentration of gallic and hydrocinnamic acid were found in Morus nigra L., vanillic, caffeic and ferulic acids were found in Glycyrrhiza glabra L. and only rosmarinic acid was found in Urtica dioica L. The highest levels of flavonoids were found in Glycyrrhiza glabra L. except for kaempferol. According to the analysis results, it can be said that Glycyrrhiza glabra L. has more effective antiradical and phycochemical effects as feed additive.
References
Abay, M., Eruygur, N. (2021). Investigation of Antioxidant Activities of Fruit, Leaf, and Branche Extracts of White (Morus alba L.) and Black (Morus nigra L.) Mulberry Species from Diyarbakır. Turk J Agric Res 2021, 8(2): 177-183. https://doi.org/10.19159/tutad.841557.
Alagawany, M., Elnesr, S.S., Farag, M.R. (2019). Use of liquorice (Glycyrrhiza glabra) in poultry nutrition: Global impacts on performance, carcass and meat quality.
World's Poultry Science Journal , 75(2),293-304. https://doi.org/10.1017/S0043933919000059
Alagawany, M., Elnesr, S.S., Farag, M.R., Abd El-Hack, M.E., Khafaga, A.F., Taha, A.E., Tiwari, R., Yatoo, M.I., Bhatt, P., Marappan, G., Dhama, K. (2019). Use of Licorice (Glycyrrhiza glabra) Herb as a Feed Additive in Poultry: Current Knowledge and Prospects. Animals, 9 (8), 536. https://doi:10.3390/ani9080536
Ashraf, A., Ashraf, M.M., Rafiqe, A., Aslam, B., Galani, S., Zafar, S., Asad, F., Asghar, R.D., Akram, S., Ahmed, H., Shah, S.M.A., Asif, R. (2017). In vivo antiviral potential of Glycyrrhiza glabra extract against Newcastle disease virus. Pak J Pharm Sci, 30(2), 567–572.
Babich, O., Ivanova, S., Ulrikh, E., Popov, A., Larina, V., Frolov, A., Prosekov. (2022). A. Study of the Chemical Composition and Biologically Active Properties of Glycyrrhiza glabra Extracts. Life, 12 (11), 1772. https://doi.org/10.3390/life12111772.
Baliyan, S., Mukherjee, R., Priyadarshini, A., Vibhuti, A., Gupta, A., Pandey, R.P., Chang, C.M. (2022). Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa. Molecules, 27, 1326. https://doi.org/10.3390/molecules27041326
Barbosa, V., Almeida, V.V., Manfron, J., Raman, V., Oliveira, C.F.D., Betim, F.C.M., Cruz, L.S., Swiech, J.N.D., Oliveira, V.B.D., Paula, J.D.F.D., Miguel, O.G. (2023). Antioxidant Activity and Determination of Phenolic Compounds, Total Flavonoids and Hispidulin in Baccharis erioclada DC. Brazilian Archives of Biology and Technology, 66(e23220459). https://doi.org/10.1590/1678-4324-2023220459
Bhusal, K.K., Magar, S.K., Thapa, R., Lamsal, A., Bhandari, S., Maharjan, R., Shrestha, S., Shrestha, J. (2022). Nutritional and pharmacological importance of stinging nettle (Urtica dioica L.): A review. Heliyon, 8 (2022) e09717, pp:1-8. https://doi.org/10.1016/j.heliyon.2022.e09717
Brand-Williams, W., Cuvelier, M.E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activ¬ity. LWT Food Sci Technol, 28 (1), 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
Ceylan, R., Ak, G., Akata, I., Zengin, G. (2021). Üç Lactarius Türünün Antioksidan Ve Enzim İnhibitör Aktiviteleri Üzerine Karşılaştırmalı Bir Çalışma. The Journal of Fungus. 12(1), 19-28. https://doi. org/10.30708.mantar.826538
Christaki, E., Giannenas, I., Bonos, E., Florou-Paneri, P. (2020). Chapter 2-Innovative uses of aromatic plants as natural supplements in nutrition. In: Florou-Paneri P, Christaki E, Giannenas I, editors. Feed Additives. London: Academic Pres (pp. 19–34. https://doi.org/10.1016/B978-0-12-814700-9.00002-9
Decker, E.A., Welch, B. (1990). Role of ferritin as a lipid oxidation catalyst in muscle food. J Agric Food Chem, 38, 674–677. https://doi.org/10.1021/jf00093a019
Dhama, K., Karthik, K., Khandia, R., Munjal, A., Tiwari, R., Rana, R., Khurana, S.K., Ullah, S., Khan, R.U., Alagawany, M., Farag, M.R., Joshi, S.K. (2018). Medicinal and therapeutic potential of herbs and plant metabolites/extracts countering viral pathogens-Current knowledge and prospects. Curr Drug Metab, 19 (3), 236–263. https://doi.org/10.2174/1389200219666180129145252
Dhama, K., Latheef, S.K., Mani, S., Samad, H.A., Karthik, K., Tiwari, R., Khan, R.U., Alagawany, M., Farag, M.R., Alam, G.M., Laudadio, V., Tufarelli, V. (2015). Multiple beneficial applications and modes of action of herbs in poultry health and production-A review. Int J Pharmaco, 11: 152-176. https://doi.org/10.3923/ijp.2015.152.176
Flórez, M., Cazón P., Vázquez, M. (2022). Antioxidant extracts of nettle (Urtica dioica) leaves: evaluation of extraction techniques and solvents. Molecules, 27 (18), 6015. https://doi.org/10.3390/molecules27186015
Franz, C.M., Baser, K.H.C., Hahn-Ramssl I. (2020). Chapter 3-Herbs and aromatic plants as feed additives: Aspects of composition, safety, and registration rules. In: Florou-Paneri P, Christaki E, Giannenas I, editors. Feed Additives. London: Academic Press (pp. 35–56). https://doi.org/10.1016/B978-0-12-814700-9.00003-0
Gao, Q., Che, N., Li, B., Zu, M., Ma, Y., Xu, H., Zhu, Z., Reis, R.L., Kundu, S.C., Xiao, B. (2024). Natural lipid nanoparticles extracted from Morus nigra L. leaves for targeted treatment of hepatocellular carcinoma via the oral route. Journal of Nanobiotechnology, 22:4. https://doi.org/10.1186/s12951-023-02286-3
Halliwell, B., Gutteridge, J.M., Aruoma, O.I. (1987). The deoxyribose method: a simple “test-tube” assay for de-termination of rate constants for reactions of hydroxyl radicals. Anal Biochem, 165(1), 215–219. https://doi. org/10.1016/0003-2697(87)90222-3
Keser, S., Demir, E., Yilmaz, Ö. (2014). Phytochemicals and antioxidant activity of the almond kernel (Prunus dulcis Mill.) from Turkey. J Chem Soc Pak, 36(3), 534-541.
Mahfuz, S., Shang, Q., Piao, X. (2021). Phenolic compounds as natural feed additives in poultry and swine diets: a review. Journal of Animal Science and Biotechnology, 12:48. pp.1-18. https://doi.org/10.1186/s40104-021-00565-3
Panche, A.N., Diwan, A.D., Chandra, S.R. (2016). Flavonoids: an overview. Journal of Nutritional Science, 5 (47), pp. 1-15. https://doi.org/10.1017/jns.2016.41
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med, 26(9–10), 1231–1237. https://doi.org/10.1016/s0891-5849(98)00315-3
Rubalya-Valantina S. Neelamegam, P. (2015). Selective ABTS and DPPH- radical scavenging activity of peroxide from vegetable oils. International Food Research Journal, 22(1), 289-294.
Salami, S.A., Guinguina. A., Agboola, J.O., Omede, A.A., Agbonlahor, A.A, Tayyab, U. (2016). Review: In vivo and postmortem effects of feed antioxidants in livestock: a review of the implications on authorization of antioxidant feed additives. Animal, 10(8), pp. 1375–1390. https://doi.org/10.1017/S1751731115002967
Semenescu, I., Avram, S., Similie, D., Minda, D., Diaconeasa, Z., Muntean, D., Lazar, A.E., Gurgus, D., Danciu, C. (2024). Phytochemical, Antioxidant, Antimicrobial and Safety Profile of Glycyrrhiza glabra L. Extract Obtained from Romania. Plants, 13(23), 3265. https://doi.org/10.3390/plants13233265
Slinkard, K., Singleton, V.L. (1977). Total phenol analy¬sis-automation and comparison with manual methods. Am J Enol Vitic, 28, 49–55. https://doi.org/10.5344/ ajev.1974.28.1.49
Sridhar, K., Charles, A.L. (2019). In vitro antioxidant activity of Kyoho grape extracts in DPPH and ABTS assays: Estimation methods for EC50 using advanced statistical programs. Food Chem, 275 (2019), pp. 41–49. https://doi.org/10.1016/j.foodchem.2018.09.040
Starcevic, K., Krstulović, L., Brozić, D., Maurić, M., Stojević, Z., Mikulec, Ž., Bajic, M., Masek, T. (2015). Production performance, meat composition and oxidative susceptibility in broiler chicken fed with different phenolic compounds. J Sci Food Agric, 95(6):1172–8. https://doi.org/ 10.1002/jsfa.6805
Sultanayeva, L., Karkehabadi, S., Zamaratskaia, G., Balji Y. (2023). Tannins and flavonoids as feed additives in the diet of ruminants to improve the performance and quality of the derived products. A review. Bulgarian Journal of Agricultural Science, 29 (3), 522–530.
Şeyhahmet, A. (2023). The effect of pH and extraction time on total phenolic and antioxidant activity of red cabbage (Brassica oleracea var.capitata f.rubra). Master's Thesis. Sakarya University, Institute of Science.
Uğur, Y., Güzel, A. (2023). Determination of phytochemical content by LC-MS/MS, investigation of antioxidant capacity, and enzyme inhibition effects of nettle (Urtica dioica). European Review for Medical and Pharmacological Sciences, 27, 1793-1800.
Vuolo, M.M., Lima, V.S., Junior, M.R.M. (2019). Chapter 2-Phenolic compounds: structure, classification, and antioxidant power. In: MRS C, editor. Bioactive Compounds. Duxford: Woodhead Publishing; 2019. p. 33–50. https://doi.org/10.1016/B978-0-12-814774-0.00002-5
Xing, T., Gao, F., Tume, R.K., Zhou, G., Xu, X. (2019). Stress effects on meat quality: A mechanistic perspective. Compr Rev Food Sci Food Saf, 18(2), pp. 380–401. https://doi.org/10.1111/1541-4337.12417
Zu, Y., Li, C., Fu, Y., Zhao, C. (2006). Simultaneous de-termination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippo¬phae rhamnoides L.) leaves by RP-HPLC with DAD. J Pharm Biomed Anal, 41(3), 714–719. https://doi. org/10.1016/j.jpba.2005.04.052
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