Influence of LDPE and HDPE Packaging with Perforations on Banana Ripening and Postharvest Quality
DOI:
https://doi.org/10.24925/turjaf.v13i10.2916-2925.7810Keywords:
Holes, PLW, Post-Harvest, Pulp-to-Peel Ratio, Ripening ChamberAbstract
Banana (Musa spp. ‘Grand Naine’) is a climacteric fruit prone to rapid postharvest deterioration, necessitating effective storage interventions. This study investigated the influence of perforated low-density polyethylene (LDPE) and high-density polyethylene (HDPE) packaging on the ripening physiology and quality attributes of bananas over ten days. A Completely Randomized Design (CRD) was used with five treatments including control, LDPE (24 and 48 holes) and HDPE (24 and 48 holes), and four replication per treatment. Fruits were exposed to ethylene (24 hours at 14–20°C, 85–90% RH) and subsequently monitored for ten days at ambient temperature. Quality attributes such as peel color, physiological loss in weight (PLW), firmness, pulp-to-peel ratio, total soluble solids (TSS), titratable acidity (TA), TSS/TA ratio, and pH were assessed. Data were analyzed using analysis of variance (ANOVA) and mean comparisons performed with Least Significant Difference (LSD) tests at a 5% significance level. LDPE with 24 perforations minimized PLW (4.56%) and delayed ripening, while HDPE 24 holes best retained firmness (0.89 kg/cm2). Although TSS and TA trends were similar across treatments, LDPE and HDPE maintained higher pH values, indicating moderated acid metabolism. The TSS/TA ratio increased over time in all treatments, consistent with progressive ripening. LDPE 24 perforations offered the most balanced microenvironment, optimizing water retention and gas exchange. These findings support its application for enhancing banana shelf life and quality in subtropical storage conditions.
References
A1 Overseas. (2025). Cavendish G9 banana exporter India. https://www.a1overseas.com/cavendish-g9-banana
Acharya, A., Joshi, S., Bhatta, R., & Bhatta, R. (2025). Effect of different packaging materials and calcium chloride on post-harvest longevity of banana. Archives of Agriculture and Environmental Science, 10(1), 132–138. https://doi.org/10.26832/24566632.2025.1001019
Adhikari, A., Paudel, B., & Pokhrel, A. (2022). Banana production status and its role on income generation and livelihood improvement in Chitwan, Nepal. International Journal of Applied Sciences and Biotechnology, 10(2), 93–98. https://doi.org/10.3126/ijasbt.v10i2.46177
Al-Dairi, M., Pathare, P. B., Al-Yahyai, R., Jayasuriya, H., & Al-Attabi, Z. (2023). Postharvest quality, technologies, and strategies to reduce losses along the supply chain of banana: A review. Trends in Food Science & Technology, 134, 177–191. https://doi.org/10.1016/j.tifs.2023.03.003
Ali, S., Khan, A. S., Malik, A. U., Anjum, M. A., Nawaz, A., & Shah, H. M. S. (2019). Modified atmosphere packaging delays enzymatic browning and maintains quality of harvested litchi fruit during low temperature storage. Scientia Horticulturae, 254, 14–20. https://doi.org/10.1016/j.scienta.2019.04.065
Aryal, S., Dhakal, S., Khanal, P., Basnet, B., & Srivastava, A. (2020). Effect of Different Number of Perforations in Modified Atmosphere Packaging on Postharvest Life of Capsicum (Capsicum annuum var Longum). Agriways, 8(2), 125–131. https://doi.org/10.38112/agw.2020.v08i02.010
Azene, M., Workneh, T. S., & Woldetsadik, K. (2011). Effect of packaging materials and storage environment on postharvest quality of papaya fruit. Journal of Food Science and Technology, 51(6), 1041–1055. https://doi.org/10.1007/s13197-011-0607-6
Bakirci, I., & Kavaz, A. (2008). An investigation of some properties of banana yogurts made with commercial ABT‐2 starter culture during storage. International Journal of Dairy Technology, 61(3), 270–276. https://doi.org/10.1111/j.1471-0307.2008.00409.x
Banda, K., Caleb, O. J., Jacobs, K., & Opara, U. L. (2015). Effect of active-modified atmosphere packaging on the respiration rate and quality of pomegranate arils (cv. Wonderful). Postharvest Biology and Technology, 109, 97–105. https://doi.org/10.1016/j.postharvbio.2015.06.002
Borges, C. V., Amorim, E. P., Leonel, M., Gomez, H. A. G., Santos, T. P. R. D., Da Silva Ledo, C. A., Belin, M. A. F., De Almeida, S. L., Minatel, I. O., & Lima, G. P. P. (2019). Post-harvest physicochemical profile and bioactive compounds of 19 bananas and plantains genotypes. Bragantia, 78(2), 284–296. https://doi.org/10.1590/1678-4499.20180252
Brizzolara, S., Manganaris, G. A., Fotopoulos, V., Watkins, C. B., & Tonutti, P. (2020). Primary metabolism in fresh fruits during storage. Frontiers in Plant Science, 11. https://doi.org/10.3389/fpls.2020.00080
Burlakoti, P. G., & Gautam, D. M. (Fruits, plantation crops and postharvest technology (1st ed.). Heritage Publishers & Distributors Pvt. Ltd.
Buthelezi, N. M. D., & Mafeo, T. P. (2024). Effect of perforated low-density polyethylene films on postharvest quality of avocado fruit. Heliyon, 10(5), e27656. https://doi.org/10.1016/j.heliyon.2024.e27656
Chauhan, O. P., Raju, P. S., Dasgupta, D. K., & Bawa, A. (2006). Modified atmosphere packaging of banana (cv. pachbale) with ethylene, carbon di-oxide and moisture scrubbers and effect on its ripening behaviour. American Journal of Food Technology, 1(2), 179–189. https://doi.org/10.3923/ajft.2006.179.189
Cho, B., & Koseki, S. (2021). Determination of banana quality indices during the ripening process at different temperatures using smartphone images and an artificial neural network. Scientia Horticulturae, 288, 110382. https://doi.org/10.1016/j.scienta.2021.110382
Dadzie, B. K., & Orchard, J. E. (1997). Routine post-harvest screening of banana/plantain hybrids: Criteria and methods (INIBAP Technical Guidelines No. 2). International Network for the Improvement of Banana and Plantain. https://hdl.handle.net/10568/104447
De Souza, A. V., Mello, J. M., Favaro, V. F. S., Santos, T. G. F., Santos, G. P., Sartori, D. L., & Putti, F. F. (2021). Metabolism of bioactive compounds and antioxidant activity in bananas during ripening. Journal of Food Processing and Preservation, 45(11). https://doi.org/10.1111/jfpp.15959
Department of Hydrology and Meteorology. (2023). Annual climate bulletin 2023. Government of Nepal.
Du, L., Huang, X., Li, Z., Qin, Z., Zhang, N., Zhai, X., Shi, J., Zhang, J., Shen, T., Zhang, R., & Wang, Y. (2025). Application of smart packaging in fruit and vegetable preservation: a review. Foods, 14(3), 447. https://doi.org/10.3390/foods14030447
Du, L., Yang, X., Song, J., Ma, Z., Zhang, Z., & Pang, X. (2014). Characterization of the stage dependency of high temperature on green ripening reveals a distinct chlorophyll degradation regulation in banana fruit. Scientia Horticulturae, 180, 139–146. https://doi.org/10.1016/j.scienta.2014.10.026
El-Ramady, H. R., Domokos-Szabolcsy, É., Abdalla, N. A., Taha, H. S., & Fári, M. (2014). Postharvest management of fruits and vegetables storage. In Sustainable agriculture reviews (pp. 65–152). Springer. https://doi.org/10.1007/978-3-319-09132-7_2
Food and Agriculture Organization of the United Nations. (1995). Prevention of post-harvest food losses: Fruits, vegetables and root crops, 2. https://www.fao.org/4/t0073e/T0073E01.htm
Fox, J., & Weisberg, S. (2019). An R companion to applied regression (3rd ed.). SAGE Publications. https://us.sagepub.com/en-us/nam/an-r-companion-to-applied-regression/book246125
Fraundorfer, J. (2022, January 11). Ripening fruit in ripening chambers: How bananas & the like ripen in the best possible way. AgriTechTomorrow. https://www.agritechtomorrow.com/article/2021/12/ripening-fruit-in-ripening-chambers-–-how-bananas-the-like-ripen-in-the-best-possible-way/13377
Gaikwad, K. K., & Rubino, M. (2015). Water vapour permeability of packaging materials (LDPE) and fabricated package systems. School of Packaging, Michigan State University. https://www.researchgate.net/profile/Abdelkader_Bouaziz/post/Do-you-have-any-information-about-WVP-standard-value-required-for-packaging-films/attachment/5f43ea1fce377e00016edab1/AS%3A928094445662208%401598286367243/download/WVP+3.pdf
Gaikwad, K. K., Singh, S., & Negi, Y. S. (2019). Ethylene scavengers for active packaging of fresh food produce. Environmental Chemistry Letters, 18(2), 269–284. https://doi.org/10.1007/s10311-019-00938-1
Ghimire, R., Yadav, P. K., Shrestha, A. K., Devkota, A. R., & Khanal, S. (2021). Ripening regulation of banana cv. Malbhog using different ripening inducers. Turkish Journal of Agriculture - Food Science and Technology, 9(12), 2113–2123. https://doi.org/10.24925/turjaf.v9i12.2113-2123.4294
Giri, D., Lamichhane, J. J., Bhandari, G., & Basnet, M. (2024). Effect of different packaging materials on banana ripening and shelf-life in Dang, Nepal. Archives of Agriculture and Environmental Science, 9(3), 436–441. https://doi.org/10.26832/24566632.2024.090305
Golding, J. B., Shearer, D., McGlasson, W. B., & Wyllie, S. G. (1999). Relationships between respiration, ethylene, and aroma production in ripening banana. Journal of Agricultural and Food Chemistry, 47(4), 1646–1651. https://doi.org/10.1021/jf980906c
Hailu, M., Workneh, T. S., & Belew, D. (2012). Effect of packaging materials on shelf life and quality of banana cultivars (Musa spp.). Journal of Food Science and Technology, 51(11), 2947–2963. https://doi.org/10.1007/s13197-012-0826-5
Herrera, K. Y., Jaramillo, J. C., Riaño, C., Suarez, C., Sierra, C. A., Zuluaga, C. M., & Castellanos, D. A. (2024). Evaluation of perforation‐mediated modified atmosphere packaging for the commercialization of bulk purple passion fruit under refrigeration. Journal of Food Science, 89(12), https://doi.org/10.1111/1750-3841.17547
Hussein, Z., Caleb, O. J., & Opara, U. L. (2015). Perforation-mediated modified atmosphere packaging of fresh and minimally processed produce—A review. Food Packaging and Shelf Life, 6, 7–20. https://doi.org/10.1016/j.fpsl.2015.08.003
Islam, M. N., Imtiaz, M. Y., Alam, S. S., Nowshad, F., Shadman, S. A., & Khan, M. S. (2018). Artificial ripening on banana (Musa Spp.) samples: Analyzing ripening agents and change in nutritional parameters. Cogent Food & Agriculture, 4(1), 1477232. https://doi.org/10.1080/23311932.2018.1477232
Kader, A. A. (2003). A perspective on postharvest horticulture (1978-2003). HortScience, 38(5), 1004–1008. https://doi.org/10.21273/hortsci.38.5.1004
Kader, A. A. (2013). Postharvest technology of horticultural crops: An overview from farm to fork. Ethiopian Journal of Applied Science and Technology, 1(1), 1-8. https://journals.ju.edu.et/index.php/ejast/article/view/537
Kays, S. J., & Paull, R. E. (2004). Postharvest biology. Exon Press, Athens. http://hdl.handle.net/10125/65829
Khalid, S., Majeed, M., Ullah, M., Shahid, M., Riasat, A. R., Abbas, T., Aatif, H. M., & Farooq, A. (2020). Effect of storage conditions and packaging material on postharvest quality attributes of strawberry. Journal of Horticultural and Postharvest Research, 3(2), 195–208. https://doi.org/10.22077/jhpr.2019.2826.1093
Kinhal, V. (2024). How does controlled atmosphere storage extend fruit shelf-life? Felix Instruments. https://felixinstruments.com/blog/how-does-controlled-atmosphere-storage-extend-fruit-shelf-life/
Klee, H. J., & Giovannoni, J. J. (2011). Genetics and control of tomato fruit ripening and quality attributes. Annual Review of Genetics, 45(1), 41–59. https://doi.org/10.1146/annurev-genet-110410-132507
Li, Y., Li, G. S., Liu, G. F., Kan, Y. M., & Wang, J. Q. (2013). Effect of preparation conditions on property of LDPE modified atmosphere packaging film of fruit and vegetable. Applied Mechanics and Materials, 469, 135–139. https://doi.org/10.4028/www.scientific.net/amm.469.135
Lohani, S., Trivedi, P. K., & Nath, P. (2004). Changes in activities of cell wall hydrolases during ethylene-induced ripening in banana: effect of 1-MCP, ABA and IAA. Postharvest Biology and Technology, 31(2), 119–126. https://doi.org/10.1016/j.postharvbio.2003.08.001
Lufu, R., Ambaw, A., & Opara, U. L. (2020). Water loss of fresh fruit: Influencing pre-harvest, harvest and postharvest factors. Scientia Horticulturae, 272, 109519. https://doi.org/10.1016/j.scienta.2020.109519
Maduwanthi, S. D. T., & Marapana, R. A. U. J. (2017). Biochemical changes during ripening of banana: A review. International Journal of Food Science and Nutrition, 2(5), 166–169. http://dr.lib.sjp.ac.lk/handle/123456789/7777
Mahajan, P. V., & Lee, D. S. (2023). Modified atmosphere and moisture condensation in packaged fresh produce: Scientific efforts and commercial success. Postharvest Biology and Technology, 198, 112235. https://doi.org/10.1016/j.postharvbio.2022.112235
Mangaraj, S., Goswami, T. K., & Mahajan, P. V. (2009). Applications of plastic films for modified atmosphere packaging of fruits and vegetables: A review. Food Engineering Reviews, 1(2), 133–158. https://doi.org/10.1007/s12393-009-9007-3
Mari, M., & Guizzardi, M. (1998). The postharvest phase: Emerging technologies for the control of fungal diseases. Phytoparasitica, 26(1), 59–66. https://doi.org/10.1007/bf02981267
McKeen, L. (2012). Introduction to use of plastics in food packaging. Plastic Films in Food Packaging (pp.1-15). Elsevier. https://doi.org/10.1016/b978-1-4557-3112-1.00001-6
Ministry of Agriculture and Livestock Development (MoALD). (2022). Statistical Information on Nepalese Agriculture 2021/22. Retrieved January 15 from https://moald.gov.np/
Mohammed, M., Wilson, L. A., & Gomes, P. I. (1999). Postharvest sensory and physiochemical attributes of processing and nonprocessing tomato cultivars. Journal of Food Quality, 22(2), 167–182. https://doi.org/10.1111/j.1745-4557.1999.tb00549.x
Paudel, B., & Regmi, S. (2024). Effects of different traditional ripening methods on quality and shelf life of bananas in Chitwan, Nepal. Archives of Agriculture and Environmental Science, 9(4), 768–773. https://doi.org/10.26832/24566632.2024.0904019
Paudel, R. K. (2020, June 29). G9 banana more disease-resistant but less marketable, farmers say. Kathmandu Post. https://kathmandupost.com/money/2020/06/29/g9-banana-more-disease-resistant-but-less-marketable-farmers-say
Paul. V., & Pandey. R. (2011). Role of internal atmosphere on fruit ripening and storability-a review. Journal of Food Science and Technology, 51(7), 1223–50. https://doi.org/10.1007/s13197-011-0583-x
Paull, R. E., & Duarte, O. (2025). Bananas and plantains. In Tropical fruits, 1, (pp. 224–272). CABI. https://doi.org/10.1079/9781800623798.0008
Pokhrel, P. (2014). Use of higher ethylene generating fruits for ripening as an alternative to ethylene. Journal of Food Science and Technology Nepal, 8, 84–86. https://doi.org/10.3126/jfstn.v8i0.11757
Prasad, R., Ram, R. B., Kumar, V., & Rajvanshi, S. K. (2015). Study on effect of different packaging materials on shelf life of banana (Musa paradisiaca L.) cv. Harichal under different conditions. International Journal of Pure and Applied Bioscience, 3(4), 132–141. https://www.ijpab.com/vol3-iss4.php
Puri, B., Chapagaee, P., Shrestha, S., Magar, B. N., & Gautam, B. (2025). Impact of packaging materials on banana shelf life and quality in subtropical Nepal. AgroEnvironmental Sustainability, 3(1), 31–39. https://doi.org/10.59983/s2025030104
Rodov, V., Porat, R., Sabag, A., Kochanek, B., & Friedman, H. (2022). Microperforated compostable packaging extends shelf life of ethylene-treated banana fruit. Foods, 11(8), 1086. https://doi.org/10.3390/foods11081086
Ruwali, A., Thakuri, M. S., Pandey, S., Mahat, J., & Shrestha, S. (2022). Effect of different ripening agents in storage life of banana (Musa paradisiaca) at Deukhuri, Dang, Nepal. Journal of Agriculture and Food Research, 10, 100416. https://doi.org/10.1016/j.jafr.2022.100416
Saltveit, M. E. (1999). Effect of ethylene on quality of fresh fruits and vegetables. Postharvest Biology and Technology, 15(3), 279–292. https://doi.org/10.1016/s0925-5214(98)00091-x
Sonawane, A. D., Chaiwong, S., Weltzien, C., & Mahajan, P. V. (2023). A model integrating fruit physiology, perforation, and scavenger for prediction of ethylene accumulation in fruit package. Postharvest Biology and Technology, 209, 112734. https://doi.org/10.1016/j.postharvbio.2023.112734
Timilsina, U., & Shrestha, A. K. (2022). Improvement of postharvest quality and shelf life of banana cv. Malbhog using different plant extracts and modified atmosphere packages in Chitwan, Nepal. Archives of Agriculture and Environmental Science, 7(2), 255–260. https://doi.org/10.26832/24566632.2022.0702014
United States Environmental Protection Agency. (1995). R.E.D. facts: Ethephon. (EPA-738-F-95-004). https://archive.epa.gov/pesticides/reregistration/web/pdf/0382fact.pdf
Wang, S., Herrera‐Balandrano, D. D., Jiang, Y., Shi, X., Chen, X., Liu, F., & Laborda, P. (2023). Application of chitosan nanoparticles in quality and preservation of postharvest fruits and vegetables: A review. Comprehensive Reviews in Food Science and Food Safety, 22(3), 1722–1762. https://doi.org/10.1111/1541-4337.13128
Waskar, D. P., Khedkar, R. M., & Garande, V. (1999). Effect of post-harvest treatments on shelf life and quality of pomegranate in evaporative cool chamber and ambient conditions. Journal of Food Science and Technology, 36(2), 114–117.
Wills, R.B.H. & Golding, J.B. (2016) Postharvest: An Introduction to the Physiology and Handling of Fruit and Vegetables. Centre for Agriculture and Bioscience International, Wallingford, Oxon; Cambridge, MA. https://doi.org/10.1079/9781786391483.0000
Yang, X., Pang, X., Xu, L., Fang, R., Huang, X., Guan, P., Lu, W., & Zhang, Z. (2009). Accumulation of soluble sugars in peel at high temperature leads to stay-green ripe banana fruit. Journal of Experimental Botany, 60(14), 4051–4062. https://doi.org/10.1093/jxb/erp238
Zenebe, W. A., Ali, M. I., Derbew, B. Y., & Tarekegn, A. W. M. (2015). Effect of traditional kerosene smoking and ethrel on ripening, shelf life and quality of Cavendish banana (Musa sp.). African Journal of Agricultural Research, 10(50), 4570–4583. https://doi.org/10.5897/ajar2015.10299
Zhu, X., Chen, J., Qiu, K., & Kuai, B. (2017). Phytohormone and light regulation of chlorophyll degradation. Frontiers in Plant Science, 8. 1911. https://doi.org/10.3389/fpls.2017.01911
Zhu, X., Zhu, Q., & Zhu, H. (2023). Editorial: Towards a better understanding of fruit ripening: Crosstalk of hormones in the regulation of fruit ripening. Frontiers in Plant Science, 14. 1173877. https://doi.org/10.3389/fpls.2023.1173877
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.


