Comparison of Extraction Techniques for Determining Bioactive Compounds and Antioxidant Activity of Spirulina platensis
DOI:
https://doi.org/10.24925/turjaf.v12i4.554-560.6677Keywords:
Spirulina platensis, freeze-thawing, antioxidant activity, phenolic compoundsAbstract
Spirulina platensis (S. platensis) is a high-nutrient blue-green algae that has been used as a food supplement for a long time. It contains carbohydrates, lipids, proteins, vitamins, minerals, and bioactive compounds essential for basic human nutrition. It is known to have anti-cancer, antioxidant, anti-inflammatory, neuroprotective, hepatoprotective, and hypocholesterolemic properties due to the bioactive compounds it contains. In this study, the effects of freeze-thawing, a rapid freezing (-20°C) and thawing (4°C) process, and ultrasonically assisted extraction techniques on the color, antioxidant capacity, total phenolic content, and phenolic composition of Spirulina platensis extracts were investigated. The antioxidant capacity of the extracts obtained was determined by two different methods, DPPH (2,2-diphenyl-1-picryl hydrazyl) and ABTS (2,2-azinobis (3-ethylbenzothiazollin-6-sulfonic acid)). The sugar profile was determined by HPLC-RID and phenolic composition was determined by HPLC-ESI-DAD-MS/MS. The antioxidant activity and total phenolic content of samples prepared by the freeze-thawing were higher than those prepared by ultrasonic-assisted conventional extraction technique. In addition to ferulic acid 4-O-glucuronide and brevifolin carboxylate, an isocoumarin derivative, as the dominant phenolic compound in S. platensis extracts, a total of 10 phenolic compounds including catechin isomer, resveratrol C-hexoside, myricetin, ferulic acid, gallic acid, phloroglucinol, and lutein were detected. Glucose was the predominant sugar in both samples. The total sugar content was higher in the freeze-thawed samples (217.92 mg/100 g DW) than in the ultrasonic-assisted conventional extraction technique (182.91 mg/100 g DW). S. platensis has a significant amount of antioxidants, valuable secondary metabolites, and potential commercial applications and medicinal properties, but releasing these compounds is difficult due to the cell wall. This study was carried out to determine how different extraction techniques alter the release of bioactive compounds.
References
Rahman, M., Ara, R. (2022) Optimization of ultrasound-assisted extraction of phenolic content & antioxidant activity of hog plum (Spondias pinnata L. f. kurz) pulp by response surface methodology. Heliyon, 8, e11109. https://doi.org/10.1016/j.heliyon.2022.e11109
Alajil Alslibi, Z. (2019). Influence of Spirulina and whey protein hydrolysate on growth rate and activity of some probiotic bacteria in ayran. Master of Science Thesis, Gaziantep University, Graduate School of Natural Sciences, Department of Biochemistry Science and Technology, Turkey.
Al-Dhabi, N. A., & Valan Arasu, M. (2016). Quantification of phytochemicals from commercial Spirulina products and their antioxidant activities. Evidence-Based Complementary and Alternative Medicine, 1-13. https://doi.org/10.1155/2016/7631864
Arranz, S., Silván, J. M., & Saura‐Calixto, F. (2010). Nonextractable polyphenols, usually ignored, are the major part of dietary polyphenols: a study on the Spanish diet. Molecular Nutrition & Food Research, 54(11), 1646-1658. https://doi.org/10.1002/mnfr.20090058
Barwick, V. (2016). Eurachem/CITAC Guide: Guide to quality in analytical chemistry: An aid to accreditation (3rd edition). Available from www.eurachem.org.
Bernatoniene, J., Kopustinskiene, D. M. (2018). The role of catechins in cellular responses to oxidative stress. Molecules, 20;23(4):965. https://doi.org/10.3390/molecules23040965
Brand-Williams, W., Cuvelier, M.E., Berset, C.L.W.T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28, 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
Caporgno, M.P., Mathys, A. (2018). Trends in microalgae incorporation into innovative food products with potential health benefits. Frontiers in Nutrition, 5, 58. https://doi.org/10.3389/fnut.2018.00058
Chaiklahan, R., Chirasuwan, N., Triratana, P., Loha, V., Tia, S., & Bunnag, B. (2013). Polysaccharide extraction from Spirulina sp. and its antioxidant capacity. International Journal of Biological Macromolecules, 58, 73–78. https://doi.org/10.1016/j.ijbiomac.2013.03.046
Choubey S, Varughese LR, Kumar V, Beniwal V. (2015). Medicinal importance of gallic acid and its ester derivatives: a patent review. Pharmaceutical patent analyst, 4(4):305-15. https://doi.org/10.4155/ppa.15.14
De la Rosa, L. A., Moreno-Escamilla, J. O., Rodrig o-García, J., & Alvarez-Parrilla, E. (2019). Phenolic compounds. Postharvest Physiology and Biochemistry of Fruits and Vegetables, 253–271. https://doi.org/10.1016/B978-0-12-813278-4.00012-9
Ferreres, F., Lopes, G., Gil-Izquierdo, A., Andrade, P. B., Sousa, C., Mouga, T., & Valentão, P. (2012). Phlorotannin extracts from fucales characterized by HPLC-DAD-ESI-MSn: approaches to hyaluronidase inhibitory capacity and antioxidant properties. Marine Drugs, 10, 2766-2781. https://doi.org/10.3390/md10122766
Gupta, S., Gupta, C., Garg, A.P., Prakash, D. (2017). Probiotic efficiency of blue-green algae on probiotics microorganisms. Journal of Microbiology and Experimentation, 4(4), 00120. https://doi.org/10.15406/jmen.2017.04.00120
Heffernan, N., Brunton, N. P., FitzGerald, R. J., & Smyth, T. J. (2015). Profiling of the molecular weight and structural isomer abundance of macroalgae-derived phlorotannins. Marine Drugs, 13, 509-528. https://doi.org/10.3390/md13010509
Kelebek, H., Jourdes, M., Selli, S., Teissedre, P.L. (2013). Comparative evaluation of the phenolic content and antioxidant capacity of sun-dried raisins. Journal of the Science of Food and Agriculture, 93(12), 2963–2972. https://doi.org/10.1002/jsfa.6125
Kuatrakul, I., Kuarthongsri, P., Yabuuchi, C., Somsai, K., Utama-ang N. (2017). Sensory descriptive analysis and physicochemical properties of Spirulina platensis from different drying processes: hot air drying and microwave vacuum drying, Current Applied Science and Technology, 17 (2).
Lee, H. S., & Coates, G. A. (2000). Quantitative study of free sugars and myo-inositol in citrus juices by HPLC and a literature compilation. Journal of Liquid Chromatography & Related Technologies, 23(14), 2123–2141. https://doi.org/10.1081/JLC-100100476
Maddiboyina, B., Vanamamalai, H.K., Roy, H., Ramaiah Gandhi, S., Kavisri, M. (2023). Food and drug industry applications of microalgae Spirulina platensis: a review. Journal of Basic Microbiology,63: 573–583. https://doi.org/10.1002/jobm.202200704
Maddina, B.Y., Asthana, G.S., Asthana, A. (2016). A review on current scenario of Spirulina drug delivery systems. World Journal of Pharmaceutical Sciences, 4: 86–9.
Martins, R., Mouro, C., Pontes, R., Nunes, J., Gouveia, I. (2023). Ultrasound-assisted extraction of bioactive pigments from Spirulina platensis in natural deep eutectic solvents. Bioresources and Bioprocessing 10. https://doi.org/10.1186/s40643-023-00692-x
Ochoa Becerra, M., Mojica Contreras, L., Hsieh Lo, M., Mateos Díaz, J., & Castillo Herrera, G. (2020). Lutein as a functional food ingredient: Stability and bioavailability. Journal of Functional Foods, 66, 103771. https://doi.org/10.1016/j.jff.2019.10377
Pietta, P. G. (2000). Flavonoids as antioxidants. Journal of Natural Products. 63 (7): 1035–42. https://doi.org/10.1021/np9904509
Purdi, T.S., Setiowati, A.D., Ningrum, A., 2023. Ultrasound-assisted extraction of Spirulina platensis protein: physicochemical characteristic and techno-functional properties. Journal of Food Measurement and Characterization 17, 5474–5486.. https://doi.org/10.1007/s11694-023-02051-y
Saafi, E.B., El Arem, A., Issaoui, M., Hammami, M., Achour, L. (2009). Phenolic content and antioxidant activity of four date palm (Phoenix dactylifera L.) fruit varieties grown in Tunisia. International Journal of Food Science & Technology, 44, 2314–2319. https://doi.org/10.1111/j.1365-2621.2009.02075.x
Saranraj, P., & Sivasakthi, S. (2014). Spirulina platensis – food for future: a review. Asian Journal of Pharmaceutical Science & Technology, 4(1), 26-33.
Seghiri, R., Kharbach, M., Essamri, A. ( 2019). Functional composition, nutritional properties, and biological activities of MoroccanSpirulinaMicroalga. Journal of Food Quality, 1–11. https://doi.org/10.1155/2019/3707219
Shahidi, F. (2015). Antioxidants. In Handbook of Antioxidants for Food Preservation, 1st ed.; Shahidi, F., Ed.; Woodhead Publishing Series in Food Science; Technology and Nutrition: Cambridge, UK, 1–14.
Shahidi, F., & Yeo, J. (2016). Insoluble-bound phenolics in food. Molecules, 21(9), 1216. https://doi.org/10.3390/molecules21091216
Tan, H.T., Khong, N.M.H., Khaw, Y.S., Ahmad, S.A., Yusoff, F.M., 2020. Optimization of the freezing-thawing method for extracting phycobiliproteins from Arthrospira sp. Molecules 25, 3894. https://doi.org/10.3390/molecules25173894
Tsuchiya H. (2001). Stereospecificity in membrane effects of catechins. Chemico-Biological Interactions, 14;134(1):41-54. https://doi.org/10.1016/s0009-2797(00)00308-2
Uzlasir, T., Selli, S., Kelebek, H. (2023). Effect of salt stress on the phenolic compounds, antioxidant capacity, microbial load, and ın vitro bioaccessibility of two microalgae species (Phaeodactylum tricornutum and Spirulina platensis). Foods, 12, 3185. https://doi.org/10.3390/foods12173185
Villarruel-López, A., Ascencio, F., Nuño, K. (2017). Microalgae, a potential natural functional food source – a review. Polish Journal of Food and Nutrition Sciences, 67(4), 251-263. https://doi.org/10.1515/pjfns-2017-0017
Wong, C. P., & Morita, H. (2019). Bacterial type III polyketide synthases. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. https://doi.org/10.1016/B978-0-12-409547-2.14640-2
Tanrıseven, D., Kadiroglu, P., Selli, S., Kelebek, H. (2020). LC-DAD-ESI MS/MS-assisted elucidation of the phenolic compounds in shalgams: Comparison of traditional and direct methods. Food Chemistry. 305,1555, doi.org/10.1016/j.foodchem.2019.125505.
Sonmezdag, A.S., Kelebek, H., Selli, S. (2019). Effect of hulling methods and roasting treatment on phenolic compounds and physicochemical properties of cultivars ‘Ohadi’ and ‘Uzun’ pistachios (Pistacia vera L.). Food Chemistry, 272, 418 426. https://doi.org/10.1016/j.foodchem.2018.08.065.
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