Molecular Survey of the Toxoplasma gondii and Neospora caninum in brain tissue of aborted fetuses of Morkaraman sheep in Muş, Türkiye

Davut Koca; Burçak Aslan Çelik; Özgür Yaşar Çelik; Adnan Ayan; Özlem Orunç Kılınç; Ali Osman Turgut; Özge Oktay Ayan

doi:10.24925/turjaf.v11i12.2407-2410.6480

Authors

Davut Koca Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Van, Türkiye https://orcid.org/0000-0002-7962-6959
Burçak Aslan Çelik Siirt University, Faculty of Veterinary Medicine, Department of Parasitology, Siirt Türkiye https://orcid.org/0000-0002-0130-970X
Özgür Yaşar Çelik Siirt University, Faculty of Veterinary Medicine, Department of Internal Medicine, Siirt Türkiye https://orcid.org/0000-0001-6365-2688
Adnan Ayan Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Genetics, Van, Türkiye https://orcid.org/0000-0002-6564-3416
Özlem Orunç Kılınç Van Yuzuncu Yil University, Van Yüzüncü Yıl University, Özalp Vocational School of Higher Education, Department of Medical Laboratory Technician, Van, Türkiye Van, Türkiye https://orcid.org/0000-0001-6233-7109
Ali Osman Turgut Siirt University, Faculty of Veterinary Medicine, Department of Animal Science, Siirt Türkiye https://orcid.org/0000-0001-6863-0939
Özge Oktay Ayan Van Yuzuncu Yil University, Faculty of Medicine, Department of Parasitology, Van, Türkiye https://orcid.org/0000-0003-2577-3774

DOI:

https://doi.org/10.24925/turjaf.v11i12.2407-2410.6480

Keywords:

Toxoplasma gondii, Neospora caninum, PCR, Sheep, Türkiye

Abstract

Toxoplasma gondii and Neospora caninum are obligate intracellular protozoan parasites that can affect different warm-blooded species worldwide. In this study, it was aimed to detect T. gondii and N. caninum using PCR method in brain tissues of aborted sheep fetuses. Brain specimens were collected from 50 Morkaraman sheep fetuses that had undergone abortion at various stages of pregnancy, within the lambing seasons of 2023 in Muş. Approximately 1 cm³ of brain tissue from the right cerebral hemisphere was excised and subsequently frozen at -20°C for DNA extraction. DNA extraction and PCR amplification were then performed. As a result of this study, 11 (22%) of 50 brain tissues were positive. All brain samples examined in this study were negative for Neospora caninum. Based on the results of this study, it is possible to say that T. gondii is an important abortion agent in sheep in this region. Although N. caninum was not detected in this study, larger scale studies are recommended. Moreover, this study provides important information to breeders and veterinarians in the field in the evaluation and management of abortion.

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Molecular Survey of the Toxoplasma gondii and Neospora caninum in brain tissue of aborted fetuses of Morkaraman sheep in Muş, Türkiye

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