Clinical Effect of Poly Herbal Unani Formulation on Dyslipidemia- A Randomized Trial




Polyherbal unani formulation, hyperlipidemia, total cholesterol, LDL, HDL, triglyceride


Background: In adults aged 30-70 with primary and moderate hyperlipidemia, the present study took place to investigate the therapeutic benefits of a polyherbal unani preparation called Garlitab. Methods: It was a prospective open label, herbal coded test drug-controlled, randomized trial. Out of total screened patients we were enrolled 212 hyperlipidemic patients of 30–70 years in the study fulfilling the inclusion criteria, we were recruited them from OPD of a hospital in Munshiganj and different Unani clinics of Dhaka, Bangladesh after obtaining written informed consent from the patients. Selected individuals were allocated into two groups at random. Group1 Received 500 mg Garlitab tablets twice daily and Group 2 received tablet atorvastatin calcium 10 mg 2 times daily. Height, weight, and blood pressure were recorded along with blood samples. The random distributions were carried out by a research assistant utilizing a random numbers table. Blood samples were taken at the beginning of the trial, 1.5 months later, and 3 months following the intervention. Results: Results for the test medication revealed a substantial drop in cholesterol levels between baseline and the data collected after three months and in case of male it was from 241.72±38.11 to 218.24±34.06 mg/dL for total cholesterol, from 198.27±30.57 to 173.54±29.34 mg/dL for LDL and from 280.78±85.81 to 207.07±51.40 mg/dL for triglyceride. HDL increases from 33.05±3.21 to 34.69±3.13 mg/dL in male patients. The control drug atorvastatin calcium also showed a significant decrease in lipids between baseline and after 3 months data and in case of male it was from 241.92±31.54 to 174.90±22.87 mg/dL for total cholesterol, from 196.20±30.91 to 130.30±24.29 mg/dL for LDL and from 279.48±115.35 to 141.27±59.55 mg/dL for triglyceride. It increases HDL from 32.00±2.25 to 34.03±2.19 mg/dL in male patients. Between the baseline and the 3-month data, the test medicine for females significantly reduced total cholesterol, LDL, and triglycerides and it was from 244.64±52.18 to 220.12±45.07 mg/dL, from 200.32±30.57 to 173.54±29.34 mg/dL and from 272.32±99.69 to 195.25±60.68 mg/dL respectively. HDL increases from 33.77±3.36 to 35.03±3.23 mg/dL. Between the baseline and the 3-month data, the control medication for females significantly reduced total cholesterol, LDL, and triglycerides and it was from 247.74±37.95 to 175.26±29.54 mg/dL, from 197.65±27.89 to 130.91±22.04 mg/dL and from 271.57±94.52 to 142.00±50.88 mg/dL respectively. It increases HDL from 32.22±2.32 to 33.46±2.94 mg/dL. Conclusions: According to the results of the study, the polyherbal formulation Garlitab can lower cholesterol levels. It may be a useful medication for treating primary hyperlipidemia.


Ajayi O, Ajayi D. 2014. Effect of dry garlic powder on plasma lipid profile and enzyme activities in some tissues of hypercholesterolemic rats. Advances in Biochemistry, 2(3), 45-49

Arsenault BJ, Rana JS, Stroes ES, Després JP, Shah PK, Kastelein JJ, Khaw KT. 2009. Beyond low-density lipoprotein cholesterol: respective contributions of non–high-density lipoprotein cholesterol levels, triglycerides, and the total cholesterol/high-density lipoprotein cholesterol ratio to coronary heart disease risk in apparently healthy men and women. Journal of the American College of Cardiology, 55(1), 35-41.

Arulmozhi DK, Kurian R, Veeranjaneyulu A, Bodhankar SL. 2007. Antidiabetic and antihyperlipidemic effects of Myristica fragrans. in animal models. Pharmaceutical Biology, 45(1), 64-68.

Ashraf R, Aamir K, Shaikh AR, Ahmed T. 2005. Effects of garlic on dyslipidemia in patients with type 2 diabetes mellitus. J Ayub Med Coll Abbottabad, 17(3), 60-64.

Aslani N, Entezari MH, Askari G, Maghsoudi Z, Maracy MR. 2016. Effect of garlic and lemon juice mixture on lipid profile and some cardiovascular risk factors in people 30-60 years old with moderate hyperlipidaemia: a randomized clinical trial. International journal of preventive medicine, 7.

Balasasirekha R, Lakshmi UK. 2012. Effect of cloves and turmeric on hyperlipidemics. Journal of Human Ecology, 37(2), 125-132.

Batool N, Ilyas N, Shabir S, Saeed M, Mazhar R, Amjid MW. 2018. A mini-review of therapeutic potential of Mangifera indica L. Pakistan Journal of Pharmaceutical Sciences, 31(4).

Block E. 2010. Allium botany and cultivation, ancient and mdern. Garlic and other alliums: the lore and the science, 1-32.

Bongiorno PB, Fratellone PM, Lo Giudice P. 2008. Potential health benefits of garlic (Allium sativum): a narrative review. Journal of Complementary and Integrative Medicine, 5(1).

Brüll V, Burak C, Stoffel-Wagner B, Wolffram S, Nickenig G, Müller C, Egert S. 2015. Effects of a quercetin-rich onion skin extract on 24 h ambulatory blood pressure and endothelial function in overweight-to-obese patients with (pre-) hypertension: a randomised double-blinded placebo-controlled cross-over trial. British journal of nutrition, 114(8), 1263-1277.

Choudhary PR, Shekhawat JS, Sharma MS, Dashora J. 2013. Effect of Allium sativum on experimentally induced hyperlipidemia in guinea pigs. Pakistan Journal of Physiology, 9(2), 38-40.

Choudhury, Kamrun Nahar AKM. 2014. Mainuddin, Mohammad Wahiduzzaman, and Sheikh Mohammed Shariful Islam. "Serum lipid profile and its association with hypertension in Bangladesh." Vascular health and risk management 2014: 327-332.

Chowdhury MZI, Haque MA, Farhana Z, Anik AM, Chowdhury AH, Haque SM, Turin TC. 2018. Prevalence of cardiovascular disease among Bangladeshi adult population: a systematic review and meta-analysis of the studies. Vascular health and risk management, 165-181.

Collins R, Reith C, Emberson J, Armitage J, Baigent C, Blackwell L, Peto R. 2016. Interpretation of the evidence for the efficacy and safety of statin therapy. The Lancet, 388(10059), 2532-2561.

Dasgupta D, Bandyopadhyay S, Sarkar N, Chakraborty M. 2021. Natural remedies for hyperlipidemia: A review. Journal of Pharmacognosy and Phytochemistry, 10(5), 181-189.

Di Angelantonio E, Sarwar N, Perry P, Kaptoge S, Ray KK, Thompson A, Danesh J. 2009. Emerging risk factors collaboration. Major lipids, apolipoproteins, and risk of vascular disease. JAMA, 302, 1993-2000.

Elkayam A, Peleg E, Grossman E, Shabtay Z, Sharabi Y. 2013. Effects of allicin on cardiovascular risk factors in spontaneously hypertensive rats. Isr Med Assoc J, 15(3), 170-3.

Ezeani NN, Edwin N, Ugwu Okechukwu PC, Ogbanshi ME. 2017. Effect of ethanol leaf extract of mangifera indica on the lipid profile of an alloxan induced diabetic albino rats. idosr journal of experimental sciences 2(1):139-163.

Food and Agriculture Organization (FAO) of the United Nations. September 1994. Archived from the original on 30 October 2018. Retrieved 29 October 2018.

Gupta E. 2020. Elucidating the phytochemical and pharmacological potential of Myristica fragrans (nutmeg). In Ethnopharmacological investigation of Indian spices (pp. 52-61). IGI global.

Gururaja GM, Mundkinajeddu D, Dethe SM, Sangli GK, Abhilash K, Agarwal A. 2015. Cholesterol esterase inhibitory activity of bioactives from leaves of Mangifera indica L. Pharmacognosy Research, 7(4), 355.

Gururaj GM, Mundkinajeddu D, Kumar AS, Dethe SM, Allan JJ, Agarwal A. 2017. Evaluation of cholesterol-lowering activity of standardized extract of Mangifera indica in albino Wistar rats. Pharmacognosy Research, 9(1), 21.

Habtemariam S. 2019. The chemical and pharmacological basis of cloves (Syzygium aromaticum (L.) Merr. & LM Perry) as potential therapy for type 2 diabetes and associated diseases. Medicinal Foods as Potential Therapies for Type-2 Diabetes and Associated Diseases; Elsevier BV: Amsterdam, The Netherlands, 551-578.

Hussien ZM. 2014. Effect of garlic on dyslipidemic patients with diabetes mellitus (Type 2). Diyala Journal of Medicine, 6(1), 100-104.

Kabir Y, Shekhar HU, Sidhu JS. 2017. Phytochemical compounds in functional properties of mangoes. Handbook of mango fruit: Production, postharvest science, processing technology and nutrition, 237-254.

Kannar D, Wattanapenpaiboon N, Savige GS, Wahlqvist ML. 2001. Hypocholesterolemic effect of an enteric-coated garlic supplement. Journal of the American College of Nutrition, 20(3), 225-231.

Kareem MA, Krushna GS, Hussain SA, Devi KL. 2009. Effect of aqueous extract of nutmeg on hyperglycaemia, hyperlipidaemia and cardiac histology associated with isoproterenol-induced myocardial infarction in rats. Tropical journal of pharmaceutical research, 8(4), 337-344.

Khoury CK, Greene S, Wiersema J, Maxted N, Jarvis A, Struik PC. 2013. An inventory of crop wild relatives of the United States. Crop Science, 53(4), 1496-1508.

Kumar KS, Bhowmik D, Chiranjib B, Tiwari P. 2010. Allium cepa: A traditional medicinal herb and its health benefits. Journal of Chemical and Pharmaceutical Research, 2(1), 283-291.

Lee KH Kim, Y H Park EJ, Hwang HJ. 2008. Effect of onion powder supplementation on lipid metabolism in high fat-cholesterol fed SD Rats. Preventive Nutrition and Food Science, 13(2), 71-76.

Legrand M, Dupuis C, Simon C. 2013. Association between systemic hemodynamics and septic acute kidney injury in critically ill patients. A retrospective observational study, 2013, 17.

Lin CY, Xue N, Zhao D, HuangX, Feng Y. (Eds.). 2016. Natural Language Understanding and Intelligent Applications: 5th CCF Conference on Natural Language Processing and Chinese Computing, NLPCC 2016, and 24th International Conference on Computer Processing of Oriental Languages, ICCPOL 2016, Kunming, China, December 2–6, 2016, Proceedings (Vol. 10102). Springer.

Maghfur RM, Shahat MM, Soliman MM. 2022. Assessment of The Therapeutic Role of Mangifera indica Leaves Extract in Diabetic Albino Rats. Egyptian Academic Journal of Biological Sciences, B. Zoology, 14(2), 247-262.

Majewski M. 2014. Allium sativum: facts and myths regarding human health. Roczniki Państwowego Zakładu Higieny, 65(1).

Maraia F, Elmhdwi I, Farag E, Elslimani FA. Saleh. MA Muftah. 2014. The prophylactic effect of oil extracted from Syzygium aromaticum on blood lipid level in mice fed high fat diet. Journal of Biochemistry Research, 2(2), 9-17.

Modi Dikshit C, Rachh Pr, Nayak Bs, Shah Bn, Modi Kp , Patel Nm, Patel Jk. 2009. Antihyperlipidemic acitivity of syzygium cumini linn. Seed extract on high cholesterol fed diet rats. Int. J. Ph. Sci. 1(2), 330-332.

Mohamed-Yassin MS, Baharudin N, Abdul-Razak S, Ramli AS, Lai NM. 2021. Global prevalence of dyslipidaemia in adult populations: a systematic review protocol. BMJ open, 11(12), e049662.

Murthy S. 2014. Hypolipidemic effects of garlic extracts in high fat high cholesterol diet fed rats. Journal of Evolution of Medical and Dental Sciences, 3(6), 1334-1339.

Nethrakere C, Nagendranayak IM, Vinodraj K, Bhat NG, Paul M, Dasaraju R. 2015. Comparison of the efficacy of clove (syzygium aromaticum) oil with pioglitazone on dexamethasone induced hepatic steatosis, dyslipidemia and hyperglycaemia in albino rats. J Clin Exp Res, 3(1), 169-73.

Noubiap JJN, Nansseu JRN, Bigna JJR, Jingi AM, Kengne AP. 2015. Prevalence and incidence of dyslipidaemia among adults in Africa: a systematic review and meta-analysis protocol. BMJ open, 5(3), e007404.

Pashapoor A, Mashhadyrafie S, Mortazavi P. 2020. The antioxidant potential and antihyperlipidemic activity of Myristica fragrans seed (nutmeg) extract in diabetic rats. Journal of Human Environment and Health Promotion, 6(2), 91-96.

Pirillo A, Casula M, Olmastroni E, Norata GD, Catapano AL. 2021. Global epidemiology of dyslipidaemias. Nature Reviews Cardiology, 18(10), 689-700.

Rahman K, Lowe GM. 2006. Garlic and cardiovascular disease: a critical review. The Journal of nutrition, 136(3), 736S-740S.

Ravnskov U, de Lorgeril M, Diamond DM, Hama R, Hamazaki T, Hammarskjöld B, Sundberg R. 2018. LDL-C (Low-Density Lipoprotein) does not cause cardiovascular disease: a comprehensive review of the current literature. Expert review of clinical pharmacology, 11(10), 959-970.

Reynertson KA, Basile MJ, Kennelly EJ. 2005. Antioxidant potential of seven myrtaceous fruits. Ethnobotany Research and Applications, 3, 025-036.

Robinson JG, Wang S, Smith BJ, Jacobson TA. 2009. Meta-analysis of the relationship between non–high-density lipoprotein cholesterol reduction and coronary heart disease risk. Journal of the American College of Cardiology, 53(4), 316-322.

Saquib N, Saquib J, Ahmed T, Khanam MA, Cullen MR. 2012. Cardiovascular diseases and type 2 diabetes in Bangladesh: a systematic review and meta-analysis of studies between 1995 and 2010. BMC public health, 12, 1-10.

Sharma S, Pathak S, Gupta G, Sharma SK, Singh L, Sharma RK, Dua K. 2017. Pharmacological evaluation of aqueous extract of syzigium cumini for its antihyperglycemic and antidyslipidemic properties in diabetic rats fed a high cholesterol diet—Role of PPARγ and PPARα. Biomedicine & Pharmacotherapy, 89, 447-453.

Sidana S, Singh VB, Meena BL, Beniwal S, Chandra S, Singh K, Kumar D. 2016. Effect of Syzygium cumini (jamun) seed powder on dyslipidemia: a double-blind randomized control trial. International Journal of Research and Medical Science, 7, 2603-2610.

Singh S, Singh L, Sagar BP, Das M. 2018. Evaluation of Antihyperlipidemic Activity of Ethanolic Extract of Syzygium cumini in Triton X-100 Induced Hyperlipidemic Rats. International Journal of Pharmacy & Pharmaceutical Research, 12(3), 40-54.

Sompong W, Muangngam N, Kongpatpharnich A, Manacharoenlarp C, Amorworasin C, Suantawee T, Adisakwattana S. 2016. The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion. BMC complementary and alternative medicine, 16(1), 1-9.

Stückle D. 2015. Towards a Paradigm Shift in Cholesterol Treatment. A Re-examination of the Cholesterol Issue in Japan: Abstracts. Annals of Nutrition and Metabolism, 66(Suppl. 4), 1-116.

Suryajayanti MF, Dewi A, Nurrahma B, Putri A, Kharia Z, Farmati A. 2017. Effect of ethanol extract of Syzygium cumini (L.) pulp on blood pressure and malondyaldehyde level of chronic restraint stress rats. J. Med. Sci.(Berkala Ilmu Kedokteran), 49, 114-120.

Vangoori Y, Dakshinamoorthi A, Kavimani S.2019. Effect of Myristica Fragrans Extract on Lipid Profile, Glucose, Body Weight, Food Intake, Liver and Renal Functions in Experimental Obese Rats. Biomedical and Pharmacology Journal, 12(2), 677-682.

Yeh YY, Liu L. 2001. Cholesterol-lowering effect of garlic extracts and organosulfur compounds: human and animal studies. The journal of nutrition, 131(3), 989S-993S.

Garlic.2023. December 19, 2022. Retrieved January 20, 2023. What to know about garlic and cholesterol. 2022. Retrieved December 23, 2022.




How to Cite

Alam, K., Sheikh, H., & Samad, M. A. (2023). Clinical Effect of Poly Herbal Unani Formulation on Dyslipidemia- A Randomized Trial. Turkish Journal of Agriculture - Food Science and Technology, 11(10), 1892–1904.



Research Paper