Vitex Agnus-Castus: Beneficial Properties, Clinical Studies, and Potential Future Applications in Food




Vitex agnus-castus, castisin, pharmacological effects, biological activity, clinical studies, food applications


Vitex agnus-castus L., commonly known as chaste tree, is a small deciduous tree whose fruits have been used for over 2500 years in ancient Egypt, Greece, Iran and Rome for various gynecological problems. Vitex, including its leaves and fruits, has been used for herbal medicinal purposes since ancient times, by being boiled and dried into fruit form. It is known that Vitex extract has anti-inflammatory, antibacterial, antifungal, antimicrobial, antioxidant, and anticancer properties. Various bioactive compounds such as iridoids, diterpenoids, flavonoids, and phenolic compounds have been identified in Vitex extract in several studies. Food supplements containing Vitex extract are mostly produced for women to improve menstruation conditions and alleviate premenstrual syndrome. In this review, functional properties, the results of clinical studies, toxicological properties, food supplements, patents, and food applications of Vitex extract are summarized.


Arokiyaraj S, Perinbam K, Agastian P, Kumar RM. 2009. Phytochemical analysis and antibacterial activity of Vitex agnus-castus. International Journal of Green Pharmacy (IJGP), 3(2): 162-164. Doi:

Arslan S, Özer CO. 2021. Hayıt tohumu (Vitex agnus-castus L.) tozunun sığır köftelerinin çeşitli kalite özellikleri ve raf ömrü üzerine etkisinin araştırılması. MSc Thesis, Fen Bilimleri Enstitüsü, Nevşehir Hacı Bektaş Veli Üniversitesi, Nevşehir, Türkiye.

Azadbakht M, Baheddini A, Shorideh SM, Naserzadeh A. 2005. Effect of Vitex agnus–castus L. leaf and fruit flavonoidal extracts on serum prolactin concentration. Journal of Medicinal Plants, 4(16): 56-61. Doi:

Barnes J. 2003. Quality, efficacy and safety of complementary medicines: fashions, facts and the future. Part II: Efficacy and safety, British Journal of Clinical Pharmacology, 55(4), 331-340. Doi:

Barrientos MAE. 2017. Obtenção, caracterização, encapsulação e aplicação do extrato de vitex (Vitex agnus castus L.). Universidade De São Paulo, Faculdade De Zootecnia Engenharia De Alimentos, Pirassununga , Brazil.

Barrientos MAE, Maeda JMK, Chaves IE, Tulini FL, de Souza VB, Thomazini M, Rodrigues CEC & Favaro‐Trindade CS. 2021. Production of vitex (Vitex agnus‐castus L.) extract in powder form using spray‐drying: Potential for the production of functional foods. Journal of Food Processing and Preservation, 45(4), e15333. Doi:

Baytop T. 1984. Türkiye’de Bitkiler ile Tedavi (Geçmiste ve Bugün). İstanbul Üniversitesi, Eczacılık Fakültesi Yayınları. 2. Baskı. No: 3255, Nobel Tıp Kitapevleri. pp. 3-4.

Berger D, Schaffner W, Schrader E, Meier B & Brattström A. 2000. Efficacy of Vitex agnus castus L. extract Ze 440 in patients with pre-menstrual syndrome (PMS). Archives of Gynecology and Obstetrics, 264(3): 150-153. Doi:

Bionorica. 2023. [Erişim Tarihi: 20.03.2023].

Blackmores. 2023. [Erişim Tarihi: 20.03.2023].

Brattström A. 2014. Use of Vitex agnus-castus Extracts for Preparing a Medicament. U.S. Patent No. US 8,637,099 B2, 28 January 2014.

Catalogue of Life 2019 Annual Checklist: Taxonomic Tree. 2019. Available online: checklist/2019/browse/tree/id/c0ad8fe8ccf5c8738376cf67784a1c03 [Erişim Tarihi: 02.2023].

Cerqueira RO, Frey BN, Leclerc E & Brietzke E. 2017. Vitex agnus castus for premenstrual syndrome and premenstrual dysphoric disorder: a systematic review. Archives of Women's Mental Health, 20(6): 713-719. Doi:

Chan EWC, Wong SK & Chan HT. 2018. Casticin from Vitex species: a short review on its anticancer and anti-inflammatory properties. Journal of Integrative Medicine, 16(3): 147-152. Doi: j.joim.2018.03.001

Corley DG, Lu Q, Ming D, Troup JP. 2002. Vitex agnus-castus Extract. Spain Patent No. ES200201072A.

Cossuta D, Simándi B, Vági E, Hohmann J, Prechl A, Lemberkovics É, Kery A & Keve T. 2008. Supercritical fluid extraction of Vitex agnus castus fruit. The Journal of Supercritical Fluids, 47(2): 188-194. Doi:

Daniele C, Thompson Coon J, Pittler MH & Ernst E. 2005. Vitex agnus castus: a systematic review of adverse events. Drug safety, 28(4), 319–332. Doi: 10.2165/00002018-200528040-00004

Dinç T & Coşkun F. 2014. Comparison of fructus agni casti and flurbiprofen in the treatment of cyclic mastalgia in premenopausal women. Ulusal Cerrahi Dergisi, 30(1): 34–38. Doi:

Dugoua JJ, Seely D, Perri D, Koren G & Mills E. 2008. Safety and efficacy of chaste tree (Vitex agnus-castus) during pregnancy and lactation. The Canadian Journal of Clinical Pharmacology, 15(1): 74-79.

Dutta AC & Dutta TC. 1964. Botany for degree students (No. 581 DUT). London: Oxford university press.

Dülger B, Uğurlu E, Gücin F. 2002. Vitex agnus-castus L. (Hayıt)’un Antimikrobiyal Aktivitesi, Çev-Kor, 11 (45): 1-5.

El Kamari F. 2019. Total Phenols Content, Flavonoids Concentration and Antioxidant Activities of Leaves Extracts of Vitex agnus-castus L. Growing Wild in Morocco. Int. J. Pharm. Sci. Research, 10(4), 1670-1676. Doi:

El Kamari F, Ousaaid D, Taroq A, El Atki Y, Aouam I, Lyoussi B & Abdellaoui A. 2021. Bioactive ingredients of different extracts of Vitex agnus-castus L. Fruits from Morocco and their antioxidant potential. Jordan Journal of Biological Sciences, 14(2): 267-270.

El-Nawasany LI. 2019. The use of Vitex Agnus-Castus to produce functional stirred yoghurt. Journal of Food and Dairy Sciences, 10(9): 297-301. Doi: 10.21608/jfds.2019.54517

Flemisch S & Wiesner J. 2018. Assessment report on Vitex agnus-castus L., fructus. Eur. Med. Agency, 44: 1-34. EMA/HMPC/606741/2018

Ghannadi A, Bagherinejad M, Abedi D, Jalali M, Absalan B & Sadeghi N. 2012. Antibacterial activity and composition of essential oils from Pelargonium graveolens L and Vitex agnus-castus L. Iranian Journal of Microbiology, 4(4): 171-176.

Girman A, Lee R & Kligler B. 2003. An integrative medicine approach to premenstrual syndrome. Am J. Obstet. Gynecol. 188: 56-65. Doi:

Gökbulut A, Ozhan O, Karacaoglu MU & Şarer E. 2010. Radical scavenging activity and vitexin content of Vitex agnus castus leaves and fruits. FABAD Journal of Pharmaceutical Sciences, 35(2): 85-91.

Gruenwald J, Brendler T. & Jaenicke C. 2007. PDR for herbal medicines. Thomson, Reuters.

Habbab A, Sekkoum K, Belboukhari N, Cheriti A & Y Aboul-Enein H. 2016. Essential oil chemical composition of Vitex agnus-castus L. from Southern-West Algeria and its antimicrobial activity. Current Bioactive Compounds, 12(1): 51-60. Doi: 666160330152633

Hajdú Z, Hohmann J, Forgo P, Martinek T, Dervarics M, Zupkó I, Falkay G, Cossuta D & Máthé I. 2007. Diterpenoids and flavonoids from the fruits of Vitex agnus-castus and antioxidant activity of the fruit extracts and their constituents. Phytother Res. 21(4): 391-4. Doi: 10.1002/ptr.2021

Hamdouch A, Chebli B, Asdadi A & Idrissi Hassani LM. 2018. Phytochemical screening, quantitative analysis and antioxidant activity of Vitex agnus castus (Lamiaceae) from South-East of Morocco. Arabian Journal of Medicinal and Aromatique Plants, 4.

Ho SH, Singh M, Holloway AC & Crankshaw DJ. 2011. The effects of commercial preparations of herbal supplements commonly used by women on the biotransformation of fluorogenic substrates by human cytochromes P450. Phytotherapy Research: PTR, 25(7): 983-989. Doi:

Hobbs C. 1991. The chaste tree: Vitex agnus castus. Pharmacy in history, 33(1): 19-24.

Ibrahim FM, Ibrahim AY, El-Newary SA, Hendawy SF & Mahomoodally MF. 2021. Vitex agnus-castus L. (Chasteberry) extracts shows in vitro and in vivo anti-inflammatory and anti-tumor propensities via reduction of cyclooxygenase-2 activity and oxidative stress complications. South African Journal of Botany, 143: 363-373. Doi:

Ibrahim N, Shalaby A, Farag R, Elbaroty G & Hassa E. 2009. Chemical composition and biological evaluation of Vitex agnus-castus L. Med Aromatic Plant Sci Biotech, 3(1), 27-31.

Jokić S, Jerković I, Rajić M, Aladić K, Bilić M & Vidović S. 2017. SC-CO2 extraction of Vitex agnus-castus L. fruits: The influence of pressure, temperature and water presoaking on the yield and GC–MS profiles of the extracts in comparison to the essential oil composition. The Journal of Supercritical Fluids, 123, 50-57. Doi: j.supflu.2016.12.007

Kamal N, Mio Asni NS, Rozlan INA, Mohd Azmi MAH, Mazlan NW, Mediani A & Edrada-Ebel RA. 2022. Traditional Medicinal Uses, Phytochemistry, Biological Properties, and Health Applications of Vitex sp. Plants, 11(15), 1944. Doi:

Kavaz A, Işık M, Dikici E & Yüksel M. 2022. Anticholinergic, Antioxidant, and Antibacterial Properties of Vitex Agnus‐Castus L. Seed Extract: Assessment of Its Phenolic Content by LC/MS/MS. Chemistry&Biodiversity, 19(10), e202200143.


Kayacık H. 1966. Orman ve Park Ağaçlarının Özel Sistematiği. 3. Cilt. Angiosperma Kapalı Tohumlular. İstanbul: İstanbul Üniversitesi Orman Fakültesi. F50 KAY 1975

Keikha N, Shafaghat M, Mousavia SM, Moudi M & Keshavarzi F. 2018. Antifungal effects of ethanolic and aqueous extracts of Vitex agnus-castus against vaginal isolates of Candida albicans. Current medical mycology, 4(1), 1. Doi:

Khalilzadeh E, Vafaei Saiah G, Hasannejad H, Ghaderi A, Ghaderi S, Hamidian G, Mahmoudi R, Eshgi D & Zangisheh M. 2015. Antinociceptive effects, acute toxicity and chemical composition of Vitex agnus-castus essential oil. Avicenna journal of phytomedicine, 5(3), 218-230. PMID: 26101755

Kırmızıbekmez H & Demir D. 2016. IriDoid Glycosides and Phenolic Compounds from the Flowers of Vitex agnus‐castus. Helvetica Chimica Acta, 99(7): 518-522. Doi:

Kikuchi H, Yuan B, Yuhara E, Imai M, Furutani R, Fukushima S, Hazama S, Hirobe C, Ohyama K, Takagi N, Toyoda H. 2014. Involvement of histone H3 phosphorylation via the activation of p38 MAPK pathway and intracellular redox status in cytotoxicity of HL-60 cells induced by Vitex agnus-castus fruit extract. International Journal of Oncology, 45: 843-852. Doi:

Kirtikar KR, Basu BD. 1935. Indian Medicinal Plants, Vol. II. Lalit Mohan Publication, Allahabad, 1347-1348.

Komlenić DK, Jukić M, Nakov G, Šušak A, Bucić-Kojić A, Planinić M, Jokić S & Lukinac J. Sensory And Qualitative Propertıes Of Wheat Flour Crackers With Chaste Tree Berry Flour (Vitex Agnus Castus) After Extraction. Journal of Hygienic Engineering & Design, 39.

Kordels, 2023. [Erişim Tarihi: 20.03.2023]

Kour M, Madom Anantharaya VN, Bhat KMR, Chakraborti S & Kodavanji B. 2017. Effect of Vitex agnus extract on MNU induced mammary tumor of Sprague Dawley rats. Journal of Young Pharmacists, 9(3): 367. Doi:

Liou CJ, Len WB, Wu SJ, Lin CF, Wu XL & Huang WC. 2014. Casticin inhibits COX-2 and iNOS expression via suppression of NF-κB and MAPK signaling in lipopolysaccharide-stimulated mouse macrophages. Journal of ethnopharmacology, 158: 310-316. Doi:

Maltaş E, Uysal A, Yildiz S & Durak Y. 2010. Evaluation of antioxidant and antimicrobial activity of Vitex agnus-castus L. Fresen Environ Bull, 19: 3094-3099.

Mancho P, Edwards QT. 2005. Chaste tree for premenstrual syndrome. An evolving therapy in the United States. Advance for Nurse Practitioners. 13: 43-4, 46. PMID: 15898315.

Mari A, Montoro P, D’Urso G, Macchia M, Pizza C & Piacente S. 2015. Metabolic profiling of Vitex agnus castus leaves, fruits and sprouts: Analysis by LC/ESI/ (QqQ) MS and (HR) LC/ESI/ (Orbitrap)/MS n. Journal of Pharmaceutical and Biomedical Analysis. 102: 215-221. Doi:

Mele A, Lentz H, Mele A, Feizlmayr E, Abazi S & Bauer R. 2013. Extraction of rotundifuran and casticin from chaste tree fruits by near critical liquid carbon dioxide. The Journal of supercritical fluids, 79, 123-126. Doi:

Mesaik MA, Murad S, Khan KM, Tareen RB, Ahmed A & Choudhary MI. 2009. Isolation and immunomodulatory properties of a flavonoid, casticin from Vitex agnus‐castus. Phytotherapy Research, 23(11): 1516-1520. Doi:

Middleton E. 1998. Effect of plant flavonoids on immune and inflammatory cell function. Advances in experimental medicine and biology, 439, 175–182. Doi:

Mirghafourvand M, Mohammad-Alizadeh-Charandabi S, Ahmadpour P. & Javadzadeh Y. 2016. Effects of Vitex agnus and Flaxseed on cyclic mastalgia: A randomized controlled trial. Complementary therapies in medicine, 24, 90–95. Doi:

Morales-Del-Rio JA, Gutiérrez-Lomelí M, Robles-García MA, Aguilar JA, Lugo-Cervantes E, Guerrero-Medina PJ, Ruiz-Cruz S, Cinco-Moroyoqui FJ, Wong-Corral FJ & Del-Toro-Sánchez CL. 2015. Anti-Inflammatory Activity and Changes in Antioxidant Properties of Leaf and Stem Extracts from Vitex mollis Kunth during In Vitro Digestion. Evidence-based complementary and alternative medicine: eCAM, 2015, 349235. Doi:

Morrow J. 2008. Composition and Method For Treatment of Premenstrual Symptoms. U.S. Patent No. US20080160083A1.

Mousavi P, Zaheri H, Najar S, Afshari P. 2015. The effect of Vitagnus on physical and emotional symptoms of premenstrual syndrome: a randomized clinical trial. Journal of Clinical Nursing and Midwifery, 4 (1) :68-76. URL:

Myasoedova VA, Kirichenko TV, Melnichenko AA, Orekhova VA, Ravani A, Poggio P, Sobenin IA, Bobryshev YV & Orekhov AN. 2016. Anti-Atherosclerotic Effects of a Phytoestrogen-Rich Herbal Preparation in Postmenopausal Women. International journal of molecular sciences, 17(8), 1318. Doi:

Niroumand MC, Heydarpour F & Farzaei MH. 2018. Pharmacological and therapeutic effects of Vitex agnus-castus L.: A review. Pharmacognosy Reviews, 12(23). Doi:

Ohyama K, Akaike T, Imai M, Toyoda H, Hirobe C & Bessho T. 2005. Human gastric signet ring carcinoma (KATO-III) cell apoptosis induced by Vitex agnus-castus fruit extract through intracellular oxidative stress. The international journal of biochemistry & cell biology, 37(7): 1496–1510. Doi:

Ono M, Eguchi K, Konoshita M, Furusawa C, Sakamoto J, Yasuda S, Ikeda T, Okawa M, Kinjo J, Yoshimitsu H & Nohara T. 2011. A new diterpenoid glucoside and two new diterpenoids from the fruit of Vitex agnus-castus. Chemical & pharmaceutical bulletin, 59(3): 392–396. Doi:

Pandey A, Bani S, Satti NK, Gupta BD & Suri KA. 2012. Anti-arthritic activity of agnuside mediated through the down-regulation of inflammatory mediators and cytokines. Inflammation Research, 61(4): 293-304. Doi:

Proestos C, Sereli D & Komaitis M. 2006. Determination of phenolic compounds in aromatic plants by RP-HPLC and GC-MS. Food chemistry, 95(1): 44-52. Doi:

Rajić M, Molnar M, Bilić M & Jokić S. 2016. The impact of extraction methods on isolation of pharmacologically active compounds from Vitex agnus-castus-a review. International Journal of Pharmaceutical Research & Allied Sciences, 5(4).

Rani A & Sharma A. 2013. The genus Vitex: A review. Pharmacognosy reviews, 7(14): 188–198. Doi:

Roemheld-Hamm B. 2005. Chasteberry. American family physician, 72(5): 821-824. PMID: 16156340.

Rossmanith WG & Ruebberdt W. 2009. What causes hot flushes? The neuroendocrine origin of vasomotor symptoms in the menopause. Gynecological endocrinology: the official journal of the International Society of Gynecological Endocrinology, 25(5): 303–314. Doi: 10.1080/09513590802632514

Saglam H & Orsal O. 2020. Effect of exercise on premenstrual symptoms: A systematic review. Complementary therapies in medicine, 48, 102272. Doi: j.ctim.2019.102272

Schellenberg R. 2001. Treatment for the premenstrual syndrome with agnus castus fruit extract: prospective, randomised, placebo-controlled study. BMJ (Clinical research ed.), 322(7279): 134–137. Doi: bmj.322.7279.134

Schellenberg R, Zimmermann C, Drewe J, Hoexter G & Zahner C. 2012. Dose-dependent efficacy of the Vitex agnus castus extract Ze 440 in patients suffering from premenstrual syndrome. Phytomedicine: International Journal of Phytotherapy and Phytopharmacology, 19(14): 1325–1331. Doi:

Singleton G. 2007. Premenstrual disorders in adolescent females: integrative management. Australian family physician, 36(8). PMID: 17676186

Solaray, 2023. [Erişim Tarihi: 20.03.2023]

Souto EB, Durazzo A, Nazhand A, Lucarini M, Zaccardelli M, Souto SB, Silva A, Severino P, Novellino E & Santini A. 2020. Vitex agnus-castus L.: Main Features and Nutraceutical Perspectives. Forests, 11, 761. Doi:

Tesch B. J. 2002. Herbs commonly used by women: an evidence-based review. Disease-a-Month, 48(10): 671-696. Doi:

Thaçi S, Krasniqi B, Cela D, Dërmaku-Sopjani M, Bahtiri E, Abazi S & Sopjani M. 2020. Mechanisms underlying the tracheorelaxant effect of Vitex agnus-castus extract. Revista Brasileira de Farmacognosia, 30(1): 103-110. Doi:

Thompsons, 2023. [Erişim Tarihi: 20.03.2023]

Tracy TS & Kingston RL (Eds.). 2007. Herbal Products: toxicology and clinical pharmacology. Springer Science & Business Media. ISBN: 1-59745-383-8

Travlos IS & Karamanos AJ. 2007. Influence of heat on seed germination and seedling emergence of chaste tree (Vitex agnus castus L.). Journal of Agronomy, 6(1), 25-28. Doi:

Tümen G, Sekendiz AD. 1989. Balıkesir ve Merkez Köylerinde Halk İlacı Olarak Kullanılan Bitkiler, Uludağ Üniversitesi Araştırma Fonu, Proje No. 86/12.

van Die MD, Burger HG, Teede HJ & Bone KM. 2013. Vitex agnus-castus extracts for female reproductive disorders: a systematic review of clinical trials. Planta medica, 79(7), 562–575. Doi:

Vimal A, Vikram L, Singhal S & Anil B. 2011. Vitex negundo: a chinese chaste tree. Int. J. Pharmaceut. Innov, 1(9). ISSN 2249-1031.

Vitex agnus-castus. Monograph. 2009. Alternative medicine review: a journal of clinical therapeutic, 14(1): 67–71. PMID: 19364195.

Yao JL, Fang SM, Liu R, Oppong MB, Liu EW, Fan GW & Zhang H. 2016. A Review on the Terpenes from Genus Vitex. Molecules (Basel, Switzerland), 21(9), 1179. Doi:

Yilar M, Bayar Y, Onaran A. 2016. Chemical Composition and Antifungal Effects of Vitex agnus-castus L. and Myrtus communis L. Plants. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 44, 466-471. Doi: nbha44210399

Zahid H, Rizwani GH & Ishaqe S. 2016. Phytopharmacological review on Vitex agnus-castus: a potential medicinal plant. Chinese Herbal Medicines, 8(1): 24-29. Doi:

Zeidabadi A, Jafari M, Emamghoreishi M, Sasani MR & Akbarzadeh M. 2022. Effect of Vitex agnus-castus and Salvia officinalis Extracts on Serum Lipids in Postmenopausal Women: An Randomized Clinical Trial. Int. J. Womens Health, 10, 1-6. Doi: ijogi.2021.19389

Zhelev I, Petkova Z, Kostova I, Damyanova S, Stoyanova A, Dimitrova-Dyulgerova I, Antova G, Ercisli S, Assouguem A, Kara M, Almeer R, Sayed AA. 2022. Chemical Composition and Antimicrobial Activity of Essential Oil of Fruits from Vitex agnus-castus L., Growing in Two Regions in Bulgaria. Plants, 11(7), 896. Doi: plants11070896




How to Cite

Altunay, C., & Taştan, Özge. (2023). Vitex Agnus-Castus: Beneficial Properties, Clinical Studies, and Potential Future Applications in Food . Turkish Journal of Agriculture - Food Science and Technology, 11(11), 2185–2193.



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