Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species

Authors

  • Muhammad Amjad Nawaz Department of Biotechnology, Chonnam National University, Yeosu, Chonnam 59626
  • Faheem Shehzad Baloch Department of Field Crops, Faculty of Agricultural and Natural Science, Abant Izzet Baysal University, 14280 Bolu
  • Hafiz Mamoon Rehman Department of Biotechnology, Chonnam National University, Yeosu, Chonnam 59626
  • Bao Le Department of Biotechnology, Chonnam National University, Yeosu, Chonnam 59626
  • Fahima Akther Department of Biotechnology, Chonnam National University, Yeosu, Chonnam 59626
  • Seung Hwan Yang Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Gyeonggi 17058
  • Chung Gyuhwa Department of Biotechnology, Chonnam National University, Yeosu, Chonnam 59626

DOI:

https://doi.org/10.24925/turjaf.v4i8.700-705.788

Keywords:

CTAB, DNA Extraction, Glycine species, Genetic analyses, PVP

Abstract

Extraction of deoxyribose nucleic acid (DNA) from plants is preliminary step in molecular biology. Fast and cost effective genomic DNA isolation from Glycine species for downstream application is a major bottleneck. Here we report a high throughput and trouble free method for genomic DNA extraction from leaf and seeds of Glycine species with high quality and quantity. Protocol reports the optimization by employing different concentrations of CTAB and PVP in extraction buffer. Efficiency of optimized protocol was compared with frequently used DNA extraction methods. Wide adoptability and utility of this protocol was confirmed by DNA extraction from leaves as well as seeds of G. max, G. soja, G. tomentella and G. latifolia. Extracted DNA was successfully subjected to PCR amplification of five microsatellite markers and four putative glycosyltransferase genes. DNA extraction protocol is reproducible, trouble free, rapid and can be adopted for plant molecular biology applications.

Author Biography

Chung Gyuhwa, Department of Biotechnology, Chonnam National University, Yeosu, Chonnam 59626

Professor, Department of Biotechnology,

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Published

15.08.2016

How to Cite

Nawaz, M. A., Baloch, F. S., Rehman, H. M., Le, B., Akther, F., Yang, S. H., & Gyuhwa, C. (2016). Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species. Turkish Journal of Agriculture - Food Science and Technology, 4(8), 700–705. https://doi.org/10.24925/turjaf.v4i8.700-705.788

Issue

Vol. 4 No. 8 (2016)

Section

Crop Production

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.