First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica)

Authors

  • Allah Bakhsh Department of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technology, Niğde University, 51240 Niğde
  • Muhammad Aasim Department of Biology, Kamil Özdağ Faculty of Sciences, Karamanoğlu Mehmetbey University, 70200 Karaman
  • Muhammad Abu Bakar Zia Department of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technology, Niğde University, 51240 Niğde
  • Muhammet Doğan Department of Biology, Kamil Özdağ Faculty of Sciences, Karamanoğlu Mehmetbey University, 70200 Karaman
  • Gökhan Sadı Department of Biology, Kamil Özdağ Faculty of Sciences, Karamanoğlu Mehmetbey University, 70200 Karaman
  • Mehmet Karataş Department of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technology, Niğde University, 51240 Niğde
  • Khalid Mahmood Khawar Department of Field Crops, Faculty of Agriculture, Ankara University, 0611 Dışkapı/Ankara

DOI:

https://doi.org/10.24925/turjaf.v4i8.642-645.747

Keywords:

Agrobacterium, Genetic transformation, Shoot tip, GUS, nptII

Abstract

The study presents first report of Agrobacterium mediated genetic transformation in Rice paddy herb (Linmophila aromatica). A. tumefaciens strain C58C1 harboring pBin19 Plasmid containing β-glucuronidase (GUS) and neomycin phosphotransferase II (nptII) gene, under the control of 35S promoter and NOS terminator was used. Shoot tip explants were inoculated for 30 min followed by co-cultivation for 72 h and selected on agar semi solidified MS medium containing 100 mg/l Kanamycin and 1.0 mg/l BA; whereas total number of 78 putative transgenic shoots were obtained. The shoots were rooted on MS medium containing 1.0 mg/l IBA and 100 mg/l Kanamycin where 43 plants survived and rooted. Expression of GUS gene in the putative transgenics was confirmed by histochemical GUS assay. Visible localised gus expression was noted in a few cells and callus tissues of 4 plantlets that were photographed using compound light microscope.

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Published

15-08-2016

How to Cite

Bakhsh, A., Aasim, M., Zia, M. A. B., Doğan, M., Sadı, G., Karataş, M., & Khawar, K. M. (2016). First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica). Turkish Journal of Agriculture - Food Science and Technology, 4(8), 642–645. https://doi.org/10.24925/turjaf.v4i8.642-645.747

Issue

Section

Crop Production