Screening Chemical Composition and Bioactive Properties of Mentha x piperita L. Essential Oil and Extract

Ilkin Sengun, Ersin Yucel, Gulden Kılıc, Berna Ozturk


In recent years, medicinal and aromatic herbs, which contain variety of antimicrobial compounds and have no risk to human health in terms of antibiotic resistance, are increasingly used as alternatives to antibiotics. In the study, chemical composition, total phenolic content, antioxidant and antimicrobial activity of essential oil and extract of Mentha x piperita L. growing wild in Eskisehir were investigated. Carvone (55.8%), limonene (12.8%), 1.8-cineole (8.7%) and trans-dihydrocarvone (6.4%) were determined as the main constituents of the essential oil, while the major components of the extract were carvone (56.4%), 1.8-cineole (14.1%) and trans-dihydrocarvone (8.4%). The total phenolic contents of essential oil (2204.33 µg GAE/g) were significantly higher than of extract (744 µg GAE/g). The essential oil and the extract were inhibited 84.08% and 42.59% by the DPPH radical, 91.87% and 48.40% by the ABTS radical, respectively. The highest inhibition zone was observed against S. aureus (23 mm). However, minimum inhibition concentration (MIC) values of the essential oil were ranged between 2.5% and 10% (v/v), while MIC values of the extract were determined as 10% and >10% (v/v). The present study demonstrated that M. piperita could be used in pharmaceutical and food applications as a natural antioxidant and antimicrobial substance.


Antibacterial activity; Antioxidant activity; Peppermint; Total phenolic content

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ISSN: 2148-127X

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