Protective Effect of Humic Substances Against Oxidative Stress Caused by Lead in Liver of Nile Tilapia, Oreochromis niloticus (Linnaeus, 1758)

Authors

  • Ferbal Özkan Yılmaz Mersin Üniversitesi, Su Ürünleri Fakültesi, Temel Bilimler Bölümü, 33160 Mersin
  • Zülfiye Su Mersin Üniversitesi, Su Ürünleri Fakültesi, Temel Bilimler Bölümü, 33160 Mersin
  • Arzu Özlüer-Hunt Mersin Üniversitesi, Su Ürünleri Fakültesi, Yetiştiricilik Bölümü, 33160 Mersin
  • Metin Yıldırım Mersin Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümü, 33160 Mersin
  • Serap Yalın Mersin Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümü, 33160 Mersin

DOI:

https://doi.org/10.24925/turjaf.v6i8.1015-1021.1914

Keywords:

Oreochromis niloticus, Lead, Humic substance, Antioxidant system, Lipid peroxidation

Abstract

In this study, the protective effect of humic substance in the fish feed was investigated against the effects of subletal lead (Pb) concentration on the antioxidant enzyme system in Oreochromis niloticus. For this purpose, the fish which were affected by 1.5 mg/L lead nitrate Pb(NO3)2 medium concentration were fed with feed containing 0.2% of humic substance. Liver samples were taken at 4th and 10th days in this study, which included control group, humic substance (HM), Pb, and Pb + HM groups. The enzyme activity of liver tissue catalase (CAT) and superoxide dismutase (SOD) of O. niloticus and the level of malondialdehyde (MDA), a product of lipid peroxidation, increased significantly at 4th and 10th days in the group with subletal lead concentration compared with the control group. Compared with Pb and HM+Pb groups, it was determined that humic substance application decreased Pb toxicity on lipid peroxidation of liver tissue and did not cause a significant change in CAT and SOD activity. According to the results of the experiment, 0.2% humic substance added to the feed reduced toxicity of subletal Pb concentration on liver tissue lipid peroxidation.

Author Biography

Ferbal Özkan Yılmaz, Mersin Üniversitesi, Su Ürünleri Fakültesi, Temel Bilimler Bölümü, 33160 Mersin

MEÜ. Su Ürünleri Fakültesi, Temel Bilimler Bölümü Öğretim Üyesi, Doç. Dr. Ferbal Özkan Yılmaz, Çukurova Üniversitesi, Fen Edebiyat Fakültesi Biyoloji Bölümü mezunuyum. Balık Fizyolojisi, Ekotoksikoloji, Biyokimya alanlarında çalışıyorum

Published

20.08.2018

How to Cite

Özkan Yılmaz, F., Su, Z., Özlüer-Hunt, A., Yıldırım, M., & Yalın, S. (2018). Protective Effect of Humic Substances Against Oxidative Stress Caused by Lead in Liver of Nile Tilapia, Oreochromis niloticus (Linnaeus, 1758). Turkish Journal of Agriculture - Food Science and Technology, 6(8), 1015–1021. https://doi.org/10.24925/turjaf.v6i8.1015-1021.1914

Issue

Section

Research Paper

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