Growth Rate, Aggressiveness, Chlorate Phenotypes and Genetic Variability of Macrophomina phaseolina Isolates from Apricot Plants

İrem Pekgöz, Fatih Mehmet Tok


Surveys were conducted in apricot orchards and gardens in Hatay province of Turkey in 2014 summer season. Apricot plants showing yellowing, wilting and root rot symptoms were collected. Infected plant tissues were surface sterilized and transferred to PDA medium which contains tetracycline. After 5-days incubation period, fungal colonies were identified based on their microscopic and macroscopic characteristics. Totally, 30 isolates were identified as Macrophomina phaseolina. All the isolates were subcultured by single microsclerotia or hyphal tip techniques and kept in +6°C room conditions. Discs of 10mm from each isolate were transferred to PDA medium and kept in 15, 20, 25, 30, 35 and 40°C temperature conditions and optimum growing temperatures were determined as 25 and 30°C. For phenotypical characterizations, all isolates were grown on minimal medium containing 120mM potassium chlorate and 21 of 30 were observed to be dense, 6 were feathery and 3 were restricted, respectively. According to results, there was no correlation between location and phenotype. For the pathogenicity tests, isolates were inoculated onto apricot, peanut, soybean, maize and melon plants and disease severity was measured by using 0-4 scale after 21-days incubation period. The observed disease severity was very high on apricot with the value of 3.87 and moderate on the other plants. There was no difference in disease severity on peanut, soybean, maize and melon seedlings statistically. For molecular characterizations, 14 RAPD primers were used and based on the analysis, 14 of 51 bands were found to be polymorphic. According to phylogenetic analysis, the isolates were grouped into 2 main clusters with many of sub-clusters. No correlation was observed between clusters and locations, temperature responses, chlorate phenotypes or pathogenicity.


Apricot; Macrophomina; Pathogenicity; Chlorate phenotype

Full Text:

PDF (Türkçe)


 Creative Commons License
This work is licensed under Creative Commons Attribution 4.0 International License

ISSN: 2148-127X

Turkish JAF Sci.Tech.

Turkish Journal of Agriculture - Food Science and Technology (TURJAF) is indexed by the following national and international scientific indexing services:





















Our Journals

Turkish Journal of Agriculture - Food Science and Technology

International Journal of Poultry - Ornamental Birds Science and Technology

Turkish Research Journal of Academic Social Science

Turkish Research Journal of Academic Technical Science

Turkish Research Journal of Academic Health and Medical Sciences

Our congresses

International Anatolian Agriculture, Food, Environment and Biology Congress (TARGID)

International Congress of the Turkish Journal of Agriculture - Food Science and Technology (TURJAF)

Our Youtube Channel


Our Books

Our Scientific Books

About Us

The Turkish Science and Technology Publishing (TURSTEP) is an online and open-access platform to publish recent research and articles of scholars worldwide. Founded in 2013 and based in Turkey, the TURSTEP as a platform for academics, educators, scholars, and students from Turkey and around the world, to connect with one another. The TURSTEP disseminates research that is proven or predicted to be of significant influence for the general public.


Contact Us

Please send all inquiries to the email:

Business Adress

Eğriköprü mh. Pilot Kaya İstektepe Cad. 9/1 BLOK Daire 7, 58050 Merkaz/Sivas, Turkey