Determination of Nuclear DNA Content of Some Spinach Accessions by Using Flow Cytometer

Authors

  • Murat Deveci Namık Kemal Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü, 59100 Tekirdağ
  • Özcan Yavaş Namık Kemal Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü, 59100 Tekirdağ
  • Nihan Sahin Namık Kemal Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü, 59100 Tekirdağ
  • Metin Tuna Namık Kemal Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, 59100 Tekirdağ

DOI:

https://doi.org/10.24925/turjaf.v6i2.239-246.1718

Keywords:

Spinacia oleracea L., Nuclear, DNA content, Flow cytometer, Ploidy, Chromosome number, Clustering analysis

Abstract

The aim of this research is to determine ploidy levels of 53 spinach (Spinacia oleracea L.) accessions obtained from abroad sources by using flow cytometry. According to the results of the statistical analysis, the differences among DNA content of accessions were statistically important. The average DNA content of spinach accessions used in the study ranged between 2.225 pg/2C (Esfenaj accession) and 2.059 pg/2C (Matador and Godir accessions). The mean nuclear DNA of accessions shown to be relatively stable due to low standard deviation (0.003-0.096). Based on the mitotic chromosome analyses of the some plants with different DNA contents, mitotic chromosome number of the all plants analyzed in the study were determined as 2n=12. These results indicate that all the accessions used in the study are diploid although their DNA content is significantly vary. According to the clustering analysis of the 2C DNA contents results, it was observed that eight sub clusters were formed under two main clusters. The clustering of axioms was performed in a similar manner to the multiple comparison test. As a result, the data will save time and labor convenience in a further breeding studies deal with the same 53 spinach accessions of our study.

Published

06.03.2018

How to Cite

Deveci, M., Yavaş, Özcan, Sahin, N., & Tuna, M. (2018). Determination of Nuclear DNA Content of Some Spinach Accessions by Using Flow Cytometer. Turkish Journal of Agriculture - Food Science and Technology, 6(2), 239–246. https://doi.org/10.24925/turjaf.v6i2.239-246.1718

Issue

Section

Crop Production