Assessment of RAPD Markers to Analyse the Genetic Diversity among Sunflower (Helianthus annuus L.) Genotypes

Authors

  • Ali Raza Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad, Pakistan.
  • Haseeb Shaukat Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad, Pakistan.
  • Qasim Ali Centre of Excellence in Molecular Biology (CEMB), University of Punjab, Lahore, Pakistan.
  • Madiha Habib Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad, Pakistan.

DOI:

https://doi.org/10.24925/turjaf.v6i1.107-111.1710

Keywords:

Diversity, PIC, RAPD, sunflower, similarity

Abstract

Genetic diversity estimation among different species is an important tool for genetic improvement to maximize the yield, desirable quality, wider adaptation, pest and insect resistance that ultimately boosting traditional plant breeding methods. The most efficient way of diversity estimation is application of molecular markers. In this study, twenty random amplified polymorphic DNA (RAPD) primers were utilized to estimate the genetic diversity between ten sunflower genotypes. Overall 227 bands were amplified by 20 primers with an average of 11.35 bands per primer. RAPD data showed 86.34% polymorophic bands and 13.65% of monomorophic bands. Genetic similarity was ranged from 50.22% to 87.22%. The lowest similarity (50.22%) was observed between FH-352 and FH-359 and the maximum similarity 87.22% was observed between A-23 and G-46. Polymorphic information content (PIC) values were varying from 0.05 to 0.12 with a mean of 0.09. Cluster analysis based on RAPD results displayed two major distinct groups 1 and 2. Group-2 contains FH-352 which was the most diverse genotype, while group-1 consists of few sub groups with all other genotypes. Ample diversity was found in all the genotypes. Present study reveals novel information about sunflower genome which can be used in future studies for sunflower improvement.

Author Biographies

Ali Raza, Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad, Pakistan.

I obtained my undergraduate degree (B.Sc. Hons.) Agriculture major Agriculture Biotechnology from Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad in 2015. Recently, I have completed my master degree (M.Sc. Hons.) Agriculture Biotechnology degree from the same institute. Which is the top 100 world ranking university and well established, research-oriented institute in the field of plant molecular biology in Pakistan.

Haseeb Shaukat, Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad, Pakistan.

I obtained my undergraduate degree (B.Sc. Hons.) Agriculture major Agriculture Biotechnology from Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad in 2015. Recently, I have completed my master degree (M.Sc. Hons.) Agriculture Biotechnology degree from the same institute. Which is the top 100 world ranking university and well established, research-oriented institute in the field of plant molecular biology in Pakistan.

Qasim Ali, Centre of Excellence in Molecular Biology (CEMB), University of Punjab, Lahore, Pakistan.

I obtained my undergraduate degree (B.Sc. Hons.) in 2015 in “Agriculture Biotechnology” from the University of Agriculture Faisalabad. I completed my Master of Philosophy (M.Phil.) degree in 2017 in “Molecular Biology” from Centre of Excellence in Molecular Biology (CEMB), University of Punjab, Pakistan.

Madiha Habib, Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad, Pakistan.

Completed my graduation in Bioinformatics from GCUF and my post-graduation in Biotechnology from UAF. Currently working on plant biotechnology (PhD Scholar) and I have great interest to learn emerging techniques of genetic engineering.

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Published

04.02.2018

How to Cite

Raza, A., Shaukat, H., Ali, Q., & Habib, M. (2018). Assessment of RAPD Markers to Analyse the Genetic Diversity among Sunflower (Helianthus annuus L.) Genotypes. Turkish Journal of Agriculture - Food Science and Technology, 6(1), 107–111. https://doi.org/10.24925/turjaf.v6i1.107-111.1710

Issue

Section

Agricultural Technologies