Effects of Plant Growth Promoting Bacteria and Auxin on Cutting Propagation of Rosa canina L.

Abstract

Rosa canina L. cuttings were obtained from the flora of Ondokuz Mayıs University campus of Kurupelit. Woody cuttings were placed into the medium of perlite and turf mixture with a ratio of 1:1 under mist propagation system with bottom heating system. Rosa canina cuttings were treated with 10 different plant growth promoting rhizobacteria (PGPR), alone or with the combination of 1000 ppm auxin (Indole-3-butyric acid-IBA), before planting into rooting medium. Cuttings were first kept in solution for 6 h and then treated with 1000 ppm IBA for 10 seconds. The rooting ratio, the diameter of rooting area, root length and the number of main roots were determined. According to the results, the highest rooting ratio was obtained from Bacillus megaterium (M-3), Bacillus megaterium (TV-60D) and Pseudomonas flourescens (TV-11D) bacteria treatments by 30% whereas rooting ratio was 10 % for control cuttings. IBA did not change the effects of some bacteria (Bacillus subtilis, Agrobacterium rubi, Paenibacillus polymyxa), slightly reduced the effects on some bacteria (Bacillus megaterium), whereas provided 10% rooting on some bacteria (Bacillus megaterium, Bacillus subtilis, Pseudomonas putida) which were not effective alone. In general, bacteria treatments improved the rooting quality (diameter of rooting area, root length and the number of primary roots) other than rooting ratio.