Antibacterial and Antioxidant Activities of Liquidambar Orientalis Mill. Various Extracts Against Bacterial Pathogens Causing Mastitis

Authors

  • Gülten Ökmen Department of Biology, Faculty of Science, Mugla Sitki Kocman University, Mugla, Turkey
  • Zafer Cantekin Department of Microbiology, Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay, Turkey
  • Mohammmad Intakhab Alam
  • Onur Türkcan Department of Biology, Faculty of Science, Mugla Sitki Kocman University, Mugla, Turkey
  • Yaşar Ergün Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay, Turkey

DOI:

https://doi.org/10.24925/turjaf.v5i8.883-887.1163

Keywords:

Antibiotic resistance, Anti-bacterial activity, Anti-oxidant activity, Liquidambar orientalis, Mastitis, Staphylococci

Abstract

Antibiotic resistance is being constantly developed worldwide. Coagulase Negative Staphylococci (CNS) and Staphylococcus aureus are common causes of bovine subclinical mastitis. Bioactive compound of medicinal plants shows anti-microbial, anti-mutagenic and anti-oxidant effects. The anti-bacterial and anti-oxidant activities of Liquidambar orientalis (L. orientalis) extracts on subclinical mastitis causing bacteria in cows have not been reported to date. The aim of the present study was to examine anti-bacterial and anti-oxidant effects of L. orientalis leaf extracts on S. aureus and CNS isolated from cows with subclinical mastitis symptoms. In this study, 3.2 mg/mL minimum inhibitory concentration (MIC) of ethanol extracts of L. orientalis has shown to be a most potent anti-bacterial and anti-oxidant for all isolated bacterial species from mastitis cows. In this study, it was investigated anti-bacterial and anti-oxidant potentials of acetone, methanol and ethanol extracts of the L. orientalis. The acetone extract showed maximum inhibition zone against S. aureus numbered 17 (12 mm). In addition to anti-bacterial properties, anti-oxidant activity of L. orientalis extract was examined by ABTS [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)] free radical assay. Trolox was used as a positive control anti-oxidant. Ethanol extract exhibited a strong anti-oxidant activity like Trolox anti-oxidant which was effective at 2.58 mM concentration. Bioactive compounds of sweet gum may be useful to screening mastitis causing bacteria for clinical applications.

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Published

26.08.2017

How to Cite

Ökmen, G., Cantekin, Z., Alam, M. I., Türkcan, O., & Ergün, Y. (2017). Antibacterial and Antioxidant Activities of Liquidambar Orientalis Mill. Various Extracts Against Bacterial Pathogens Causing Mastitis. Turkish Journal of Agriculture - Food Science and Technology, 5(8), 883–887. https://doi.org/10.24925/turjaf.v5i8.883-887.1163

Issue

Section

Animal Production